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Scientific article
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English

Characterization of the mammalian RNA exonuclease 5/NEF-sp as a testis-specific nuclear 3′ → 5′ exoribonuclease

Published inRNA, vol. 23, no. 9, p. 1385-1392
Publication date2017-09-24
First online date2017-05-24
Abstract

Ribonucleases catalyze maturation of functional RNAs or mediate degradation of cellular transcripts, activities that are critical for gene expression control. Here we identify a previously uncharacterized mammalian nuclease family member NEF-sp (RNA exonuclease 5 [REXO5] or LOC81691) as a testis-specific factor. Recombinant human NEF-sp demonstrates a divalent metal ion-dependent 3′ → 5′ exoribonuclease activity. This activity is specific to single-stranded RNA substrates and is independent of their length. The presence of a 2′- O -methyl modification at the 3′ end of the RNA substrate is inhibitory. Ectopically expressed NEF-sp localizes to the nucleolar/nuclear compartment in mammalian cell cultures and this is dependent on an amino-terminal nuclear localization signal. Finally, mice lacking NEF-sp are viable and display normal fertility, likely indicating overlapping functions with other nucleases. Taken together, our study provides the first biochemical and genetic exploration of the role of the NEF-sp exoribonuclease in the mammalian genome.

eng
Keywords
  • 2610020H08Rik
  • LOC81691
  • NEF-sp
  • Q96IC2
  • REXO5
  • REXON
  • RNA exonuclease 5
Funding
  • National Institutes of Health - Functions of MOV10L1 in piRNA biogenesis and germ cell development [1R01HD069592-01A1]
  • NICHD NIH HHS - [R01 HD069592]
Citation (ISO format)
SILVA, Sara, HOMOLKA, David, PILLAI, Ramesh. Characterization of the mammalian RNA exonuclease 5/NEF-sp as a testis-specific nuclear 3′ → 5′ exoribonuclease. In: RNA, 2017, vol. 23, n° 9, p. 1385–1392. doi: 10.1261/rna.060723.117
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ISSN of the journal1355-8382
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