Characterization of the mammalian RNA exonuclease 5/NEF-sp as a testis-specific nuclear 3′ → 5′ exoribonuclease

Article Article scientifique Article Characterization of the mammalian RNA exonuclease 5/NEF-sp as a testis-specific nuclear 3′ → 5′ exoribonuclease eng 2017-05-24 2017-09-24 author Silva Sara 645611 author Homolka David 856314 Département de biologie moléculaire et cellulaire (MOCEL) author Pillai Ramesh 854560 Département de biologie moléculaire et cellulaire (MOCEL) 1385-1392 10.1261/rna.060723.117 28539487 1355-8382 PMC5558908 http://rnajournal.cshlp.org/lookup/doi/10.1261/rna.060723.117 <p> Ribonucleases catalyze maturation of functional RNAs or mediate degradation of cellular transcripts, activities that are critical for gene expression control. Here we identify a previously uncharacterized mammalian nuclease family member NEF-sp (RNA exonuclease 5 [REXO5] or LOC81691) as a testis-specific factor. Recombinant human NEF-sp demonstrates a divalent metal ion-dependent 3′ → 5′ exoribonuclease activity. This activity is specific to single-stranded RNA substrates and is independent of their length. The presence of a 2′- O -methyl modification at the 3′ end of the RNA substrate is inhibitory. Ectopically expressed NEF-sp localizes to the nucleolar/nuclear compartment in mammalian cell cultures and this is dependent on an amino-terminal nuclear localization signal. Finally, mice lacking NEF-sp are viable and display normal fertility, likely indicating overlapping functions with other nucleases. Taken together, our study provides the first biochemical and genetic exploration of the role of the NEF-sp exoribonuclease in the mammalian genome. </p> 2610020H08Rik LOC81691 NEF-sp Q96IC2 REXO5 REXON RNA exonuclease 5 Dewey 570 National Institutes of Health Functions of MOV10L1 in piRNA biogenesis and germ cell development 1R01HD069592-01A1 US EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT NICHD NIH HHS R01 HD069592 43 Département de biologie moléculaire 161 false RNA 23 9 d5b20207-0a8e-4f98-9fd7-cf372a9aa07c Article (Published version) Public Article-scientifique-from-unpaywall.pdf 6281935 application/pdf CC BY-4.0 e6c61a58768134ccde3f2a536991652811563f56dfc42e95082f5d3068325329 openAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Characterization of the mammalian RNA exonuclease 5/NEF-sp as a testis-specific nuclear 3′ → 5′ exoribonuclease	RNA			2017	69	27

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Silva, Sara