Scientific article
Open access

A helical inner scaffold provides a structural basis for centriole cohesion

Published inScience advances, vol. 6, no. 7, p. 1-12; eaaz4137
Publication date2020-02-14

The ninefold radial arrangement of microtubule triplets (MTTs) is the hallmark of the centriole, a conserved organelle crucial for the formation of centrosomes and cilia. Although strong cohesion between MTTs is critical to resist forces applied by ciliary beating and the mitotic spindle, how the centriole maintains its structural integrity is not known. Using cryo–electron tomography and subtomogram averaging of centrioles from four evolutionarily distant species, we found that MTTs are bound together by a helical inner scaffold covering ~70% of the centriole length that maintains MTTs cohesion under compressive forces. Ultrastructure Expansion Microscopy (U-ExM) indicated that POC5, POC1B, FAM161A, and Centrin-2 localize to the scaffold structure along the inner wall of the centriole MTTs. Moreover, we established that these four proteins interact with each other to form a complex that binds microtubules. Together, our results provide a structural and molecular basis for centriole cohesion and geometry.

Citation (ISO format)
LE GUENNEC, Maeva et al. A helical inner scaffold provides a structural basis for centriole cohesion. In: Science advances, 2020, vol. 6, n° 7, p. 1–12. doi: 10.1126/sciadv.aaz4137
Main files (1)
Article (Published version)
ISSN of the journal2375-2548

Technical informations

Creation02/16/2024 12:27:38 PM
First validation02/23/2024 2:49:08 PM
Update time02/23/2024 2:49:08 PM
Status update02/23/2024 2:49:08 PM
Last indexation05/06/2024 6:00:07 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack