Dictyostelium discoideum is an amoeba that lives in the soil and feeds on bacteria. This model organism can be used as a surrogate macrophage to study highly conserved processes such as cell motility, phagocytosis, membrane traffic and host-pathogen interactions. This thesis addresses the role of the class I myosin MyoB, Dynamin A and actin dynamics in membrane traffic during phagosome maturation in Dictyostelium.