Scientific article

Engineering chromosomes in mice through targeted meiotic recombination (TAMERE)

Published inNature genetics, vol. 20, no. 4, p. 381-384
Publication date1998-12

Functional studies of large transcription units, clustered genes and chromosomal loci require the design of novel experimental tools to engineer genomic macro-rearrangements. Here, we present a strategy to produce deficiencies or duplications by crossing mice carrying loxP sites in homologous loci. This trans -allelic targeted meiotic recombination (TAMERE) protocol allows for the combination of various alleles within a particular locus as well as for generation of interchromosomal unequal exchanges. Novel genetic configurations can thus be produced without multiple targeting and selection steps in embryonic stem (ES) cells. A concomitant deletion/duplication event of the Hoxd12 locus shows the potential of this approach. The high frequency of such targeted exchanges in vivo makes TAMERE a powerful genetic tool applicable to research areas in which complex genomic modifications are required.

Citation (ISO format)
HERAULT, Yann et al. Engineering chromosomes in mice through targeted meiotic recombination (TAMERE). In: Nature genetics, 1998, vol. 20, n° 4, p. 381–384. doi: 10.1038/3861
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Article (Published version)
ISSN of the journal1061-4036

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