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Scientific article
Letter
English

Engineering chromosomes in mice through targeted meiotic recombination (TAMERE)

Published inNature genetics, vol. 20, no. 4, p. 381-384
Publication date1998-12
Abstract

Functional studies of large transcription units, clustered genes and chromosomal loci require the design of novel experimental tools to engineer genomic macro-rearrangements. Here, we present a strategy to produce deficiencies or duplications by crossing mice carrying loxP sites in homologous loci. This trans -allelic targeted meiotic recombination (TAMERE) protocol allows for the combination of various alleles within a particular locus as well as for generation of interchromosomal unequal exchanges. Novel genetic configurations can thus be produced without multiple targeting and selection steps in embryonic stem (ES) cells. A concomitant deletion/duplication event of the Hoxd12 locus shows the potential of this approach. The high frequency of such targeted exchanges in vivo makes TAMERE a powerful genetic tool applicable to research areas in which complex genomic modifications are required.

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Citation (ISO format)
HERAULT, Yann et al. Engineering chromosomes in mice through targeted meiotic recombination (TAMERE). In: Nature genetics, 1998, vol. 20, n° 4, p. 381–384. doi: 10.1038/3861
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ISSN of the journal1061-4036
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