Scientific article
Open access

Single-cell transcriptomic profiling redefines the origin and specification of early adrenogonadal progenitors

Published inCell reports, vol. 42, no. 3, 112191
Publication date2023-03-28
First online date2023-03-01

Adrenal cortex and gonads represent the two major steroidogenic organs in mammals. Both tissues are considered to share a common developmental origin characterized by the expression of Nr5a1/Sf1. The precise origin of adrenogonadal progenitors and the processes driving differentiation toward the adrenal or gonadal fate remain, however, elusive. Here, we provide a comprehensive single-cell transcriptomic atlas of early mouse adrenogonadal development including 52 cell types belonging to twelve major cell lineages. Trajectory reconstruction reveals that adrenogonadal cells emerge from the lateral plate rather than the intermediate mesoderm. Surprisingly, we find that gonadal and adrenal fates have already diverged prior to Nr5a1 expression. Finally, lineage separation into gonadal and adrenal fates involves canonical versus non-canonical Wnt signaling and differential expression of Hox patterning genes. Thus, our study provides important insights into the molecular programs of adrenal and gonadal fate choice and will be a valuable resource for further research into adrenogonadal ontogenesis.

  • CP: Developmental biology
  • Nr5a1/Sf1
  • Adrenal gland
  • Adrenogonadal primordium
  • Cell fate decision
  • Gonad
  • Mesoderm
  • Single-cell transcriptomic
  • Mice
  • Animals
  • Transcriptome / genetics
  • Gonads / metabolism
  • Cell Lineage / genetics
  • Cell Differentiation / genetics
  • Gene Expression Profiling
  • Mammals
Citation (ISO format)
NEIRIJNCK, Yasmine et al. Single-cell transcriptomic profiling redefines the origin and specification of early adrenogonadal progenitors. In: Cell reports, 2023, vol. 42, n° 3, p. 112191. doi: 10.1016/j.celrep.2023.112191
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ISSN of the journal2211-1247

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