Scientific article
Open access

Longitudinal Detection of Twenty DNA and RNA Viruses in Allogeneic Hematopoietic Stem Cell Transplant Recipients Plasma

Published inViruses, vol. 15, no. 4, 928
Publication date2023-04-07
First online date2023-04-07

Metagenomics revealed novel and routinely overlooked viruses, representing sources of unrecognized infections after allogeneic hematopoietic stem cell transplantation (allo-HSCT). We aim to describe DNA and RNA virus prevalence and kinetics in allo-HSCT recipients’ plasma for one year post HSCT. We included 109 adult patients with first allo-HSCT from 1 March 2017 to 31 January 2019 in this observational cohort study. Seventeen DNA and three RNA viral species were screened with qualitative and/or quantitative r(RT)-PCR assays using plasma samples collected at 0, 1, 3, 6, and 12 months post HSCT. TTV infected 97% of patients, followed by HPgV-1 (prevalence: 26–36%). TTV (median 3.29 × 105 copies/mL) and HPgV-1 (median 1.18 × 106 copies/mL) viral loads peaked at month 3. At least one Polyomaviridae virus (BKPyV, JCPyV, MCPyV, HPyV6/7) was detected in >10% of patients. HPyV6 and HPyV7 prevalence reached 27% and 12% at month 3; CMV prevalence reached 27%. HSV, VZV, EBV, HHV-7, HAdV and B19V prevalence remained <5%. HPyV9, TSPyV, HBoV, EV and HPg-V2 were never detected. At month 3, 72% of patients had co-infections. TTV and HPgV-1 infections were highly prevalent. BKPyV, MCPyV and HPyV6/7 were frequently detected relative to classical culprits. Further investigation is needed into associations between these viral infections and immune reconstitution or clinical outcomes.

  • PCR
  • TTV
  • Anellovirus
  • Blood
  • Human pegivirus
  • Polyomavirus
  • Transplantation
  • Virome
  • Virus
Citation (ISO format)
ZANELLA, Marie-Céline et al. Longitudinal Detection of Twenty DNA and RNA Viruses in Allogeneic Hematopoietic Stem Cell Transplant Recipients Plasma. In: Viruses, 2023, vol. 15, n° 4, p. 928. doi: 10.3390/v15040928
Main files (1)
Article (Published version)
ISSN of the journal1999-4915

Technical informations

Creation05/26/2023 11:01:07 AM
First validation06/05/2023 10:24:31 AM
Update time06/05/2023 10:24:31 AM
Status update06/05/2023 10:24:31 AM
Last indexation08/31/2023 11:42:31 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack