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Scientific article
Open access
English

Diagnostic test accuracy of an automated device for the MALDI target preparation for microbial identification

Publication date2023-02
First online date2022-12-05
Abstract

The objective of this study was to evaluate the performance of the Copan Colibrí™ against the manual preparation of the MALDI targets. We analyzed 416 (31 different species) non-duplicate strains covering the most important species identified in clinical routine. We also assessed the intra-strain repeatability between the comparable methods. We then analyzed the performance of this new method after implementation in routine on 12,253 aerobic bacterial isolates and yeasts, encompassing a total of 42 different species. Among the 416 strains analyzed, 6.3% (26/416) and 10.8% (45/416) had a score value < 2 when processed by the Colibri™ and manual method, respectively. Only 5.9% (9/152) of the Gram positive rods and cocci had a score values < 2 by the Colibri™ versus 20.4% (31/152) by the manual method. We confirmed that this relative superiority observed for the Colibri™ was due primarily in the use of the formic acid protocol. For the Gram-negative bacteria, the results of both methods were comparable; 6.6% (17/256) and 4.7% (12/256) had a score value < 2 by the Colibri™ and the manual method, respectively. After implementation in routine, the results according to the Biotyper score cut-off values were distributed as follows: < 1.70: 2.5% (304/12,253), 1.70–1.79: 1.9% (227/12,253), 1.80–1.89: 3.1% (377/12,253), 1.90–1.99: 6.7% (825/12,253), and ≥ 2: 85.9% (10,520/12,253). The Colibrí™ coupled to MALDI-TOF/MS revealed good performances and higher intra-strain repeatability as compared to the manual preparation of the MALDI targets.

eng
Keywords
  • Automation
  • Copan Colibrí™
  • MALDI-TOF/MS
  • WASPLab®
Citation (ISO format)
CHERKAOUI, Abdessalam et al. Diagnostic test accuracy of an automated device for the MALDI target preparation for microbial identification. In: European journal of clinical microbiology & infectious diseases, 2023, vol. 42, n° 2, p. 153–159. doi: 10.1007/s10096-022-04531-3
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Identifiers
ISSN of the journal0934-9723
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Technical informations

Creation01/05/2023 1:15:15 PM
First validation05/22/2023 3:19:30 PM
Update time05/22/2023 4:00:53 PM
Status update05/22/2023 4:00:53 PM
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