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Scientific article
English

Ganoderma lucidum extract promotes tumor cell pyroptosis and inhibits metastasis in breast cancer

Published inFood and chemical toxicology, vol. 174, 113654
Publication date2023-04
First online date2023-02-08
Abstract

Regulation of tumor cell death is a fundamental mechanism for tumor treatment. However, most tumors are resistant to cell death. Triggering inflammatory cell death, pyroptosis, may provide a new view of enhancing tumor cell death. Here we report a new role of Ganoderma lucidum extract (GLE) in pyroptotic cell death. Treatment with GLE (50-200 μg/mL) significantly elevated reactive oxygen species (ROS) levels and caused pyroptotic cell death in breast cancer cells. Mechanistically, GLE activates caspase 3 and further cleaves the gasdermin E (GSDME) protein to form pores on the cell membrane, releasing massive amounts of inflammatory factors in breast cancer cells. We also showed that GLE enhanced antitumor immune responses by substantially increasing the subsets of natural killer (NK) and CD8+T cells in the peripheral immune system and tumor microenvironment. In addition, GLE destroys multiple steps of tumor metastasis, including adhesion, migration, invasion, colonization, and angiogenesis. Collectively, these results suggest that GLE provides a potential approach for breast cancer treatment, which may complement chemotherapy or immunotherapy for cancer metastasis.

eng
Keywords
  • Cytotoxic lymphocytes
  • Ganoderma lucidum extract
  • Gasdermin E
  • Pyroptotic cell death
  • Tumor metastasis
  • Humans
  • Female
  • Breast Neoplasms
  • Reishi
  • Pyroptosis
  • Tumor Microenvironment
Citation (ISO format)
ZHONG, Chunlian et al. Ganoderma lucidum extract promotes tumor cell pyroptosis and inhibits metastasis in breast cancer. In: Food and chemical toxicology, 2023, vol. 174, p. 113654. doi: 10.1016/j.fct.2023.113654
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ISSN of the journal0278-6915
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