The use of porcine liver (2 → 3)-α-sialyltransferase in the large-scale synthesis of α-Neup5Ac-(2 → 3)-β-d-Galp-(1 → 3)-d-GlcpNAc, the epitope of the tumor-associated carbohydrate antigen CA 50

Lubineau, André; Augé, Claudine; Francois, Patrice

doi:10.1016/s0008-6215(00)90555-x

Scientific article

English

The use of porcine liver (2 → 3)-α-sialyltransferase in the large-scale synthesis of α-Neup5Ac-(2 → 3)-β-d-Galp-(1 → 3)-d-GlcpNAc, the epitope of the tumor-associated carbohydrate antigen CA 50

ContributorsLubineau, André; Augé, Claudine; Francois, Patrice

Published inCarbohydrate research, vol. 228, no. 1, p. 137-144

Publication date1992-04-10

Abstract

α-Neup5Ac-(2 » 3)-β-d-Galp(1 » 3)-d-GlcpNAc (2) and, α-Neup5Ac-(2 » 3)-β-d-Galp-(1 » 3)-β-d- GlcpNAcOMBn were prepared on a large scale by the action of β-d-GalpNAC (2 » 3)α-sialyltransferase (partially purified from porcine liver) on β-d-Galp-(1 » 3)-d-GlcpNAc and β-dGalp (1 » 3)-β-d-GlcpNAcOMBn, respectively. The trisaccharide 2 is the epitope of the tumor-associated carbohydrate antigen CA 50, highly expressed in human pancreatic adenocarcinoma.

Keywords

Animals
Antigens, Tumor-Associated, Carbohydrate / chemistry
Carbohydrate Sequence
Epitopes / chemistry
Humans
Liver / enzymology
Molecular Sequence Data
Sialyltransferases / isolation & purification
Sialyltransferases / metabolism
Substrate Specificity
Swine
Trisaccharides / biosynthesis

Affiliation entities

Not a UNIGE publication

Research groups

Analyse génomique et fonctionnelle du staphylocoque doré (604)

Citation (ISO format)

LUBINEAU, André, AUGÉ, Claudine, FRANCOIS, Patrice. The use of porcine liver (2 → 3)-α-sialyltransferase in the large-scale synthesis of α-Neup5Ac-(2 → 3)-β-d-Galp-(1 → 3)-d-GlcpNAc, the epitope of the tumor-associated carbohydrate antigen CA 50. In: Carbohydrate research, 1992, vol. 228, n° 1, p. 137–144. doi: 10.1016/s0008-6215(00)90555-x

Article (Published version)

Identifiers

PID : unige:166968
DOI : 10.1016/s0008-6215(00)90555-x
PMID : 1381278

Commercial URLhttps://linkinghub.elsevier.com/retrieve/pii/S000862150090555X

Journal ISSN0008-6215

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The use of porcine liver (2 → 3)-α-sialyltransferase in the large-scale synthesis of α-Neup5Ac-(2 → 3)-β-d-Galp-(1 → 3)-d-GlcpNAc, the epitope of the tumor-associated carbohydrate antigen CA 50

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