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Scientific article
English

The use of porcine liver (2 → 3)-α-sialyltransferase in the large-scale synthesis of α-Neup5Ac-(2 → 3)-β-d-Galp-(1 → 3)-d-GlcpNAc, the epitope of the tumor-associated carbohydrate antigen CA 50

Published inCarbohydrate research, vol. 228, no. 1, p. 137-144
Publication date1992-04-10
Abstract

α-Neup5Ac-(2 » 3)-β-d-Galp(1 » 3)-d-GlcpNAc (2) and, α-Neup5Ac-(2 » 3)-β-d-Galp-(1 » 3)-β-d- GlcpNAcOMBn were prepared on a large scale by the action of β-d-GalpNAC (2 » 3)α-sialyltransferase (partially purified from porcine liver) on β-d-Galp-(1 » 3)-d-GlcpNAc and β-dGalp (1 » 3)-β-d-GlcpNAcOMBn, respectively. The trisaccharide 2 is the epitope of the tumor-associated carbohydrate antigen CA 50, highly expressed in human pancreatic adenocarcinoma.

eng
Keywords
  • Animals
  • Antigens, Tumor-Associated, Carbohydrate / chemistry
  • Carbohydrate Sequence
  • Epitopes / chemistry
  • Humans
  • Liver / enzymology
  • Molecular Sequence Data
  • Sialyltransferases / isolation & purification
  • Sialyltransferases / metabolism
  • Substrate Specificity
  • Swine
  • Trisaccharides / biosynthesis
Affiliation Not a UNIGE publication
Citation (ISO format)
LUBINEAU, André, AUGÉ, Claudine, FRANCOIS, Patrice. The use of porcine liver (2 → 3)-α-sialyltransferase in the large-scale synthesis of α-Neu<i>p</i>5Ac-(2 → 3)-β-d-Gal<i>p</i>-(1 → 3)-d-Glc<i>p</i>NAc, the epitope of the tumor-associated carbohydrate antigen CA 50. In: Carbohydrate research, 1992, vol. 228, n° 1, p. 137–144. doi: 10.1016/s0008-6215(00)90555-x
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ISSN of the journal0008-6215
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