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Scientific article
Open access
English

Single-cell immune repertoire sequencing of B and T cells in murine models of infection and autoimmunity

Published inGenes and immunity, vol. 23, no. 6, p. 183-195
Publication date2022-09
First online date2022-08-26
Abstract

Adaptive immune repertoires are composed by the ensemble of B and T-cell receptors within an individual, reflecting both past and current immune responses. Recent advances in single-cell sequencing enable recovery of the complete adaptive immune receptor sequences in addition to transcriptional information. Here, we recovered transcriptome and immune repertoire information for polyclonal T follicular helper cells following lymphocytic choriomeningitis virus (LCMV) infection, CD8+ T cells with binding specificity restricted to two distinct LCMV peptides, and B and T cells isolated from the nervous system in the context of experimental autoimmune encephalomyelitis. We could relate clonal expansion, germline gene usage, and clonal convergence to cell phenotypes spanning activation, memory, naive, antibody secretion, T-cell inflation, and regulation. Together, this dataset provides a resource for immunologists that can be integrated with future single-cell immune repertoire and transcriptome sequencing datasets.

eng
Keywords
  • Animals
  • Autoimmunity
  • CD8-Positive T-Lymphocytes
  • Disease Models, Animal
  • Lymphocytic Choriomeningitis / genetics
  • Mice
  • Mice, Inbred C57BL
  • Peptides
  • Receptors, Antigen, T-Cell / genetics
Citation (ISO format)
SHLESINGER, Danielle et al. Single-cell immune repertoire sequencing of B and T cells in murine models of infection and autoimmunity. In: Genes and immunity, 2022, vol. 23, n° 6, p. 183–195. doi: 10.1038/s41435-022-00180-w
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ISSN of the journal1466-4879
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