Scientific article
Open access

Chemical-Biology-derived in vivo Sensors: Past, Present, and Future

Published inChimia, vol. 75, no. 12, 1017
Publication date2021-12-09
First online date2021-12-09

To understand the complex biochemistry and biophysics of biological systems, one needs to be able to monitor local concentrations of molecules, physical properties of macromolecular assemblies and activation status of signaling pathways, in real time, within single cells, and at high spatio-temporal resolution. Here we look at the tools that have been / are being / need to be provided by chemical biology to address these challenges. In particular, we highlight the utility of molecular probes that help to better measure mechanical forces and flux through key signalling pathways. Chemical biology can be used to both build biosensors to visualize, but also actuators to perturb biological processes. An emergent theme is the possibility to multiplex measurements of multiple cellular processes. Advances in microscopy automation now allow us to acquire datasets for 1000’s of cells. This produces high dimensional datasets that require computer vision approaches that automate image analysis. The high dimensionality of these datasets are often not immediately accessible to human intuition, and, similarly to ‘omics technologies, require statistical approaches for their exploitation. The field of biosensor imaging is therefore experiencing a multidisciplinary transition that will enable it to realize its full potential as a tool to provide a deeper appreciation of cell physiology.

Research group
Citation (ISO format)
LOEWITH, Robbie Joséph, ROUX, Aurélien, PERTZ, Olivier. Chemical-Biology-derived in vivo Sensors: Past, Present, and Future. In: Chimia, 2021, vol. 75, n° 12, p. 1017. doi: 10.2533/chimia.2021.1017
Main files (1)
Article (Published version)
ISSN of the journal0009-4293

Technical informations

Creation01/10/2023 12:34:00 PM
First validation01/10/2023 12:34:00 PM
Update time03/16/2023 10:24:20 AM
Status update03/16/2023 10:24:19 AM
Last indexation02/01/2024 9:24:39 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack