en
Scientific article
Open access
English

Infectious viral load in unvaccinated and vaccinated individuals infected with ancestral, Delta or Omicron SARS-CoV-2

Published inNature medicine, vol. 28, no. 7, p. 1491-1500
Publication date2022-07
First online date2022-04-08
Abstract

Infectious viral load (VL) expelled as droplets and aerosols by infected individuals partly determines transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). RNA VL measured by qRT-PCR is only a weak proxy for infectiousness. Studies on the kinetics of infectious VL are important to understand the mechanisms behind the different transmissibility of SARS-CoV-2 variants and the effect of vaccination on transmission, which allows guidance of public health measures. In this study, we quantified infectious VL in individuals infected with SARS-CoV-2 during the first five symptomatic days by in vitro culturability assay in unvaccinated or vaccinated individuals infected with pre-variant of concern (pre-VOC) SARS-CoV-2, Delta or Omicron BA.1. Unvaccinated individuals infected with pre-VOC SARS-CoV-2 had lower infectious VL than Delta-infected unvaccinated individuals. Full vaccination (defined as >2 weeks after receipt of the second dose during the primary vaccination series) significantly reduced infectious VL for Delta breakthrough cases compared to unvaccinated individuals. For Omicron BA.1 breakthrough cases, reduced infectious VL was observed only in boosted but not in fully vaccinated individuals compared to unvaccinated individuals. In addition, infectious VL was lower in fully vaccinated Omicron BA.1-infected individuals compared to fully vaccinated Delta-infected individuals, suggesting that mechanisms other than increased infectious VL contribute to the high infectiousness of SARS-CoV-2 Omicron BA.1. Our findings indicate that vaccines may lower transmission risk and, therefore, have a public health benefit beyond the individual protection from severe disease.

eng
Keywords
  • COVID-19
  • Humans
  • SARS-CoV-2
  • Serologic Tests
  • Viral Load
Citation (ISO format)
PUHACH, Olha et al. Infectious viral load in unvaccinated and vaccinated individuals infected with ancestral, Delta or Omicron SARS-CoV-2. In: Nature medicine, 2022, vol. 28, n° 7, p. 1491–1500. doi: 10.1038/s41591-022-01816-0
Main files (1)
Article (Published version)
accessLevelPublic
Secondary files (3)
Identifiers
ISSN of the journal1078-8956
152views
52downloads

Technical informations

Creation08/24/2022 9:48:00 AM
First validation08/24/2022 9:48:00 AM
Update time03/16/2023 8:57:40 AM
Status update03/16/2023 8:57:37 AM
Last indexation05/06/2024 2:36:27 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack