Scientific article
Open access

Generation of human islet cell type-specific identity genesets

Published inNature communications, vol. 13, no. 1, 2020
Publication date2022-04-19
First online date2022-04-19

Generation of surrogate cells with stable functional identities is crucial for developing cell-based therapies. Efforts to produce insulin-secreting replacement cells to treat diabetes require reliable tools to assess islet cellular identity. Here, we conduct a thorough single-cell transcriptomics meta-analysis to identify robustly expressed markers used to build genesets describing the identity of human α-, β-, γ- and δ-cells. These genesets define islet cellular identities better than previously published genesets. We show their efficacy to outline cell identity changes and unravel some of their underlying genetic mechanisms, whether during embryonic pancreas development or in experimental setups aiming at developing glucose-responsive insulin-secreting cells, such as pluripotent stem-cell differentiation or in adult islet cell reprogramming protocols. These islet cell type-specific genesets represent valuable tools that accurately benchmark gain and loss in islet cell identity traits.

Citation (ISO format)
VAN GURP, Léon et al. Generation of human islet cell type-specific identity genesets. In: Nature communications, 2022, vol. 13, n° 1, p. 2020. doi: 10.1038/s41467-022-29588-8
Main files (1)
Article (Published version)
ISSN of the journal2041-1723

Technical informations

Creation04/29/2022 8:50:00 AM
First validation04/29/2022 8:50:00 AM
Update time03/16/2023 6:30:49 AM
Status update03/16/2023 6:30:48 AM
Last indexation08/31/2023 8:23:54 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack