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Scientific article
Open access
English

Combining recombinase polymerase amplification and DNA-templated reaction for SARS-CoV-2 sensing with dual fluorescence and lateral flow assay output

Published inBiopolymers, vol. 113, no. 4, e23485
Publication date2022-04
First online date2022-01-13
Abstract

The early phase of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic was exacerbated by a diagnostic challenge of unprecedented magnitude. In the absence of effective therapeutics or vaccines, breaking the chain of transmission through early disease detection and patient isolation was the only means to control the growing pandemic. While polymerase chain reaction (PCR)-based methods and rapid-antigen tests rose to the occasion, the analytical challenge of rapid and sequence-specific nucleic acid-sensing at a point-of-care or home setting stimulated intense developments. Herein we report a method that combines recombinase polymerase amplification and a DNA-templated reaction to achieve a dual readout with either fluorescence (microtiter plate) or naked eye (lateral flow assay: LFA) detection. The nucleic acid templated reaction is based on an SNAr that simultaneously transfers biotin from one Peptide Nucleic Acid (PNA) strand to another PNA strand, enabling LFA detection while uncaging a coumarin for fluorescence readout. This methodology has been applied to the detection of a DNA or RNA sequence uniquely attributed to the SARS-CoV-2.

eng
Keywords
  • DNA templated reaction
  • PNA
  • RPA
  • Lateral flow assay
  • Nucleic acid sensing
Research group
Citation (ISO format)
FARRERA SOLER, Lluc et al. Combining recombinase polymerase amplification and DNA-templated reaction for SARS-CoV-2 sensing with dual fluorescence and lateral flow assay output. In: Biopolymers, 2022, vol. 113, n° 4, p. e23485. doi: 10.1002/bip.23485
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Article (Published version)
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ISSN of the journal0006-3525
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Technical informations

Creation04/26/2022 11:53:00 AM
First validation04/26/2022 11:53:00 AM
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