Doctoral thesis

Expanding TOR Signaling by Phospho-proteogenomic Analysis of Saccharomyces cerevisiae Strain TB50a

Number of pages164
Imprimatur date2022-02-18
Defense date2022-02-10

The Target of Ramapycin (TOR) pathway controls cell growth in response to environmental stresses and nutrient availability. Mammalian TOR (mTOR) is implicated in several disorders, including cancer, obesity and diabetes. A better understanding of TOR may allow novel strategies in the treatment for mTOR-related diseases. In the present work we aimed to find new players in the TOR signaling pathways in the model yeast Saccharomyces cerevisiae. We have produced a high quality, whole genome sequence of Saccharomyces cerevisiae TB50, a strain used in our lab with a specific rapamycin-sensitive phenotype that makes it a perfect tool to study TOR signaling in yeast. Variant analysis and pooled-linkage analysis allowed us to pinpoint the causative variant responsible for this phenotype, a single missense mutation in the gene MKT1. Additionally, state-of-the-art quantitative phosphoproteomics with an allosteric inhibitor of TOR, rapamycin, and an ATP-competitive inhibitor, CMB4563, identified several potential TOR targets containing rapamycin-insensitive phosphosites.

  • TOR
  • Mass spectrometry
  • TORC
  • Phosphoproteomics
  • Proteomics
  • Bioinformatics
  • Pooled-linkage analysis
Research group
Citation (ISO format)
MARTIN CAMPOS, Maria Trinidad. Expanding TOR Signaling by Phospho-proteogenomic Analysis of Saccharomyces cerevisiae Strain TB50a. 2022. doi: 10.13097/archive-ouverte/unige:159754
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Technical informations

Creation03/21/2022 3:25:00 PM
First validation03/21/2022 3:25:00 PM
Update time03/12/2024 4:20:21 PM
Status update03/12/2024 4:20:21 PM
Last indexation05/06/2024 10:28:33 AM
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