en
Doctoral thesis
English

Expanding TOR Signaling by Phospho-proteogenomic Analysis of Saccharomyces cerevisiae Strain TB50a

Number of pages164
Imprimatur date2022-02-18
Defense date2022-02-10
Abstract

The Target of Ramapycin (TOR) pathway controls cell growth in response to environmental stresses and nutrient availability. Mammalian TOR (mTOR) is implicated in several disorders, including cancer, obesity and diabetes. A better understanding of TOR may allow novel strategies in the treatment for mTOR-related diseases. In the present work we aimed to find new players in the TOR signaling pathways in the model yeast Saccharomyces cerevisiae. We have produced a high quality, whole genome sequence of Saccharomyces cerevisiae TB50, a strain used in our lab with a specific rapamycin-sensitive phenotype that makes it a perfect tool to study TOR signaling in yeast. Variant analysis and pooled-linkage analysis allowed us to pinpoint the causative variant responsible for this phenotype, a single missense mutation in the gene MKT1. Additionally, state-of-the-art quantitative phosphoproteomics with an allosteric inhibitor of TOR, rapamycin, and an ATP-competitive inhibitor, CMB4563, identified several potential TOR targets containing rapamycin-insensitive phosphosites.

eng
Keywords
  • TOR
  • Mass spectrometry
  • TORC
  • Phosphoproteomics
  • Proteomics
  • Bioinformatics
  • Pooled-linkage analysis
Research group
Citation (ISO format)
MARTIN CAMPOS, Maria Trinidad. Expanding TOR Signaling by Phospho-proteogenomic Analysis of Saccharomyces cerevisiae Strain TB50a. 2022. doi: 10.13097/archive-ouverte/unige:159754
Main files (1)
Thesis
accessLevelRestricted
Identifiers
317views
9downloads

Technical informations

Creation03/21/2022 3:25:00 PM
First validation03/21/2022 3:25:00 PM
Update time03/12/2024 4:20:21 PM
Status update03/12/2024 4:20:21 PM
Last indexation05/06/2024 10:28:33 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack