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Enforced tethering elongates the cortical endoplasmic reticulum and limits store-operated calcium entry

Published in Journal of cell science. 2022
Abstract Recruitment of STIM proteins to cortical ER (cER) domains forming membrane contact sites (MCS) mediate the store-operated Ca2+ entry (SOCE) pathway essential for human immunity. The cER is dynamically regulated by STIM and tethering proteins during SOCE, but the ultrastructural rearrangement and functional consequences of cER remodelling are unknown. Here, we express natural (E-Syt1/2) and artificial (MAPPER-S/L) protein tethers in HEK-293T cells and correlate the changes in cER length and gap distance measured by electron microscopy with ionic fluxes. Native cER cisternae extended during store depletion and remained elongated at constant ER-PM gap distance during subsequent Ca2+ elevations. Tethering proteins enhanced store-dependent cER expansion, anchoring the enlarged cER at tether-specific gap distances of 12-15nm (E-Syts) and 5-9nm (MAPPERs). Cells with artificially extended cER had reduced SOCE and reduced agonist-induced Ca2+ release. SOCE remained modulated by calmodulin and exhibited enhanced Ca2+-dependent inhibition. We propose that cER expansion mediated by ER-PM tethering at a close distance negatively regulates SOCE by confining STIM-ORAI complexes to the periphery of enlarged cER sheets, a process that might participate in the termination of store-operated Ca2+ entry.
Keywords Calcium signalingElectron microscopyInnate immunityIon channelsMembrane contact sitesMuscle physiology
PMID: 35191477
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Article (Accepted version) (2.7 MB) - public document Free access
Research group Signaux intracellulaires (210)
Swiss National Science Foundation: 310030_189042
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HENRY, Christopher, CARRERAS SUREDA, Amado, DEMAUREX, Nicolas. Enforced tethering elongates the cortical endoplasmic reticulum and limits store-operated calcium entry. In: Journal of cell science, 2022. doi: 10.1242/jcs.259313 https://archive-ouverte.unige.ch/unige:159487

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Deposited on : 2022-03-11

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