Intact protein analysis in the biopharmaceutical field

Staub Sporri, Aline; Guillarme, Davy; Schappler, Julie; Veuthey, Jean-Luc; Rudaz, Serge

doi:10.1016/j.jpba.2011.01.031

Scientific article

English

Intact protein analysis in the biopharmaceutical field

Contributors

Staub Sporri, Aline; Guillarme, Davy; Schappler, Julie; Veuthey, Jean-Luc; Rudaz, Serge

Published in

Journal of pharmaceutical and biomedical analysis, vol. 55, no. 4, p. 810-822

Publication date

2011

Abstract

In recent years, a growing number of biopharmaceutical proteins have been produced and are already available, or will be soon available, in the market. These molecules are more complex to analyze than conventional low molecular weight drugs, and thus need powerful analytical approaches for the entire development and delivery process. This review summarizes the analytical techniques available for intact protein determination and the main development steps in which they are applicable. A strong emphasis has been put on separation techniques, liquid chromatography and electrophoretic techniques, but mass spectrometry and spectroscopic approaches are also mentioned. Overall, we highlight how several analytical strategies are necessary to obtain global information.

Keywords

Intact protein
Biopharmaceutical analysis
Analytical methods
Liquid chromatography
Capillary electrophoresis

UNIGE affiliation entities

Faculté des sciences / Section des sciences pharmaceutiques

Citation (ISO format)

STAUB SPORRI, Aline et al. Intact protein analysis in the biopharmaceutical field. In: Journal of pharmaceutical and biomedical analysis, 2011, vol. 55, n° 4, p. 810–822. doi: 10.1016/j.jpba.2011.01.031

Article (Accepted version)

Identifiers

PID : unige:15799
DOI : 10.1016/j.jpba.2011.01.031
PMID : 21334842

Additional URL for this publication

http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TGX-5223Y1K-6-1&_cdi=5266&_user=9565874&_pii=S0731708511000562&_origin=gateway&_coverDate=06%2F25%2F2011&_sk=999449995&view=c&wchp=dGLzVtb-zSkWA&md5=17f2f95450282f0c51bf3f6255c079ec&ie=/sdarticle.pdf

Journal ISSN

1873-264X

665views

3downloads

Creation

11/05/2011 08:32:00

First validation

11/05/2011 08:32:00

Update

14/03/2023 16:51:31

Status update

14/03/2023 16:51:31

Last indexation

18/03/2025 09:20:40

Intact protein analysis in the biopharmaceutical field

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation