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Title

Niche specialization and spread of Staphylococcus capitis involved in neonatal sepsis

Authors
Wirth, Thierry
Bergot, Marine
Rasigade, Jean-Philippe
Pichon, Bruno
Barbier, Maxime
Martins-Simoes, Patricia
Jacob, Laurent
Pike, Rachel
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CollaborationWith : Schrenzel, Jacques
Published in Nature microbiology. 2020, vol. 5, no. 5, p. 735-745
Abstract The multidrug-resistant Staphylococcus capitis NRCS-A clone is responsible for sepsis in preterm infants in neonatal intensive care units (NICUs) worldwide. Here, to retrace the spread of this clone and to identify drivers of its specific success, we investigated a representative collection of 250 S. capitis isolates from adults and newborns. Bayesian analyses confirmed the spread of the NRCS-A clone and enabled us to date its emergence in the late 1960s and its expansion during the 1980s, coinciding with the establishment of NICUs and the increasing use of vancomycin in these units, respectively. This dynamic was accompanied by the acquisition of mutations in antimicrobial resistance- and bacteriocin-encoding genes. Furthermore, combined statistical tools and a genome-wide association study convergently point to vancomycin resistance as a major driver of NRCS-A success. We also identified another S. capitis subclade (alpha clade) that emerged independently, showing parallel evolution towards NICU specialization and non-susceptibility to vancomycin, indicating convergent evolution in NICU-associated pathogens. These findings illustrate how the broad use of antibiotics can repeatedly lead initially commensal drug-susceptible bacteria to evolve into multidrug-resistant clones that are able to successfully spread worldwide and become pathogenic for highly vulnerable patients.
Keywords AdultAnti-Bacterial Agents/pharmacologyBayes TheoremDrug Resistance, Multiple, Bacterial/drug effects/geneticsFranceGenes, Bacterial/geneticsGenome, BacterialGenome-Wide Association StudyGenotypeHumansInfantInfant, NewbornIntensive Care Units, NeonatalMicrobial Sensitivity TestsMutationNeonatal Sepsis/microbiologyPhenotypePolymorphism, Single NucleotideRecombination, GeneticStaphylococcal Infections/microbiologyStaphylococcus capitis/drug effects/genetics/isolation & purification/pathogenicityVancomycin/therapeutic use
Identifiers
PMID: 32341568
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Article (Published version) (5.4 MB) - document accessible for UNIGE members only Limited access to UNIGE
Other version: https://hal.archives-ouvertes.fr/hal-03056509
Research group Analyse génomique et fonctionnelle du staphylocoque doré (604)
Citation
(ISO format)
WIRTH, Thierry et al. Niche specialization and spread of Staphylococcus capitis involved in neonatal sepsis. In: Nature Microbiology, 2020, vol. 5, n° 5, p. 735-745. doi: 10.1038/s41564-020-0676-2 https://archive-ouverte.unige.ch/unige:157583

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Deposited on : 2021-12-22

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