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Disruption of vacuolin microdomains in the host Dictyostelium discoideum increases resistance to Mycobacterium marinum -induced membrane damage and infection

First online date2021-11-16
Abstract

Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, manipulates the host phagosome maturation pathway to replicate intracellularly. Mycobacterium marinum , a closely-related species, and Dictyostelium discoideum , a social amoeba and alternative phagocytic host, have been used as models to study host-pathogen interactions occurring during mycobacterial infections. Vacuolins, functional homologues of the mammalian flotillins, organize membrane microdomains and play a role in vesicular trafficking. Various pathogens have been reported to manipulate their membrane association and function. During infection of D . discoideum with M . marinum , Vacuolin C was specifically and highly induced and all three vacuolin isoforms were enriched at the mycobacteria-containing-vacuole (MCV). In addition, absence of vacuolins reduced escape from the MCV and conferred resistance to M . marinum infection. Moreover, ESAT-6, the membrane-disrupting virulence factor of M . marinum , was less associated with membranes when vacuolins were absent. Together, these results suggest that vacuolins are important host factors that are manipulated by mycobacteria to inflict membrane damage and escape from their compartment.

NoteDéposé dans: bioRxiv
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Citation (ISO format)
BOSMANI, Cristina et al. Disruption of vacuolin microdomains in the host Dictyostelium discoideum increases resistance to Mycobacterium marinum -induced membrane damage and infection. 2021. doi: 10.1101/2021.11.16.468763
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