Ovarian cancer is the seventh most common cancer in women worldwide, accounting for more deaths than any other cancer of the female reproductive system. In most of the cases, ovarian cancer will be diagnosed at the advanced stages, when cancer is already widely metastasized, often creating undetectable microscopic tumor spots that represent a major risk for disease progression, as a complete resection is rarely possible with the existing techniques. This thesis aimed at proposing a novel and innovative solution not only for diagnosis, but also for treatment planning, surgery guidance and post-resection validation for patients with ovarian cancer. The main goal was to develop a hybrid imaging tool specific for ovarian cancer with folate as targeting moiety, a chelator coordinating Ga-68 for PET imaging and fluorescent dye for surgery guidance. The probe was succesfully synthetized and demonstrated good performance on ovarian cancer cell model in vitro and in ovo.