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Characterisation of metal uptake systems in Pseudomonas aeruginosa

Defense date2021
Abstract

It is well-known that P. aeruginosa owns an ABC transporter, called ZnuABC, to translocate the zinc from the periplasm to the cytoplasm of the cell (Gonzalez et al., 2019). Recent studies suggest that other genes are involved in zinc uptake, such as the PA2911-PA2914 and PA4063-PA4066 operons coding for two additional ABC transporters (Pederick et al., 2015). Here, we take an interest in the role of the different permeases, which are PA4065, PA2914 and ZnuB. In this study we will use a combination of strains lacking one or more of these proteins to investigate their involvement in zinc uptake, in bacterial virulence, and in antibiotic resistance. Then we will particularly look into the PA4063-PA4066 operon since the ΔPA4065 mutant showed the most important growth defect when exposed to zinc-depleted medium (M-LB). Moreover, this operon appears to have two genes, PA4063 and PA4066, that encode for solute-binding proteins, suggesting more than one metal specificity. In order to explore this last hypothesis, we will purify both periplasmic proteins and try to determine their metal properties.

eng
Keywords
  • Pseudomonas aeruginosa
  • Zinc
  • Metal uptake systems
  • PA4063-PA4066 operon
Citation (ISO format)
MARTIN- -PELAUD, Oriane. Characterisation of metal uptake systems in Pseudomonas aeruginosa. 2021.
Main files (1)
Master thesis
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Identifiers
  • PID : unige:155030
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Creation09/16/2021 12:43:00 PM
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