Global Analysis of the Zinc Homeostasis Network in Pseudomonas aeruginosa and Its Gene Expression Dynamics

Article Article scientifique Article Global Analysis of the Zinc Homeostasis Network in Pseudomonas aeruginosa and Its Gene Expression Dynamics eng 2021-10-08 2021-10-08 author Ducret Verena 596806 author Abdou Melina 918860 author Goncalves Milho Catarina 852878 author Leoni Sara 745429 author Martin- -Pelaud Oriane 857935 author Sandoz Antoine 809371 author Segovia Campos Inès 892639 author Tercier Waeber Marie-Louise 0000-0002-5984-7930 22565 author Valentini Martina 0000-0002-1948-8339 889251 author Perron Karl 45679 739988 10.3389/fmicb.2021.739988 34690984 1664-302X PMC8531726 https://www.frontiersin.org/articles/10.3389/fmicb.2021.739988/full Zinc is one of the most important trace elements for life and its deficiency, like its excess, can be fatal. In the bacterial opportunistic pathogen Pseudomonas aeruginosa , Zn homeostasis is not only required for survival, but also for virulence and antibiotic resistance. Thus, the bacterium possesses multiple Zn import/export/storage systems. In this work, we determine the expression dynamics of the entire P. aeruginosa Zn homeostasis network at both transcript and protein levels. Precisely, we followed the switch from a Zn-deficient environment, mimicking the initial immune strategy to counteract bacterial infections, to a Zn-rich environment, representing the phagocyte metal boost used to eliminate an engulfed pathogen. Thanks to the use of the NanoString technology, we timed the global silencing of Zn import systems and the orchestrated induction of Zn export systems. We show that the induction of Zn export systems is hierarchically organized as a function of their impact on Zn homeostasis. Moreover, we identify PA2807 as a novel Zn resistance component in P. aeruginosa and highlight new regulatory links among Zn-homeostasis systems. Altogether, this work unveils a sophisticated and adaptive homeostasis network, which complexity is key in determining a pathogen spread in the environment and during host-colonization. NanoString Pseudomonas aeruginosa Carbapenem Homeostasis Zinc Dewey 540 Dewey 550 Dewey 580 Dewey 615 Dewey 616 Swiss National Science Foundation Zinc homeostasis in Pseudomonas aeruginosa: deciphering the role of Zur as a master regulator of metal import and export 31003A_179336 CH Project funding Swiss National Science Foundation RNA-binding proteins as multifaceted regulators of Pseudomonas aeruginosa lifestyles PZ00P3_174063 CH Careers Gebert Rüf Stiftung (Basel, Switzerland) Project Microbials GRS-071/17 Fundação para a Ciência e Tecnologia CEECIND/01777/2018 40 Département de chimie minérale, analytique et appliquée 145 42 Département des sciences de la Terre 154 45 Département des sciences végétales 163 31 Section des sciences pharmaceutiques 17 54 Département de microbiologie et médecine moléculaire 21B false 772 Groupe Bakker https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE183060 https://figshare.com/collections/Global_Analysis_of_the_Zinc_Homeostasis_Network_in_Pseudomonas_aeruginosa_and_Its_Gene_Expression_Dynamics/5653345 Frontiers in microbiology 12 f47d13c4-735e-48bb-8794-0fbe7d082b2b Article (Published version) Public Article-scientifique-from-unpaywall.pdf 3276363 application/pdf CC BY-4.0 openAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie minérale, analytique et appliquée Faculté des sciences / Section des sciences de la Terre et de l'Environnement / Département des sciences de la Terre Faculté des sciences / Section de biologie / Département des sciences végétales Faculté des sciences / Section des sciences pharmaceutiques Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

Title	Published in	Year	Views	Downloads

Global Analysis of the Zinc Homeostasis Network in Pseudomonas aeruginosa and Its Gene Expression Dynamics	Frontiers in microbiology	2021	231	288
Characterisation of metal uptake systems in Pseudomonas aeruginosa		2021	80	2

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