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Title

Unmasking viral sequences by metagenomic next-generation sequencing in adult human blood samples during steroid-refractory/dependent graft-versus-host disease

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Published in Microbiome. 2021, vol. 9, no. 1, 28
Abstract Viral infections are common complications following allogeneic hematopoietic stem cell transplantation (allo-HSCT). Allo-HSCT recipients with steroid-refractory/dependent graft-versus-host disease (GvHD) are highly immunosuppressed and are more vulnerable to infections with weakly pathogenic or commensal viruses. Here, twenty-five adult allo-HSCT recipients from 2016 to 2019 with acute or chronic steroid-refractory/dependent GvHD were enrolled in a prospective cohort at Geneva University Hospitals. We performed metagenomics next-generation sequencing (mNGS) analysis using a validated pipeline and de novo analysis on pooled routine plasma samples collected throughout the period of intensive steroid treatment or second-line GvHD therapy to identify weakly pathogenic, commensal, and unexpected viruses.
Keywords AdultAgedGraft vs Host Disease/blood/virologyHigh-Throughput Nucleotide SequencingHumansMaleMetagenomicsMiddle AgedProspective StudiesSteroids/adverse effects/therapeutic useYoung Adult
Identifiers
PMID: 33487167
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Article (Published version) (676 Kb) - public document Free access
Supplemental data (144 Kb) - public document Free access
Structures
Research groups Groupe Laurent Kaiser (virologie clinique) (668)
Leucémie et transplantation allogénique de cellules souches hématopoïétiques (982)
Lymphomes MALT et réplication virale (807)
Quorom-sensing et gènes de virulence (301)
Project
Swiss National Science Foundation: 320030_179507
Citation
(ISO format)
ZANELLA, Marie-Céline et al. Unmasking viral sequences by metagenomic next-generation sequencing in adult human blood samples during steroid-refractory/dependent graft-versus-host disease. In: Microbiome, 2021, vol. 9, n° 1, p. 28. doi: 10.1186/s40168-020-00953-3 https://archive-ouverte.unige.ch/unige:154099

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Deposited on : 2021-08-24

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