Scientific Article
previous document  unige:154070  next document
add to browser collection
Title

Predicting therapeutic nanomedicine efficacy using a companion magnetic resonance imaging nanoparticle

Authors
Miller, Miles A
Gadde, Suresh
Pfirschke, Christina
Engblom, Camilla
Sprachman, Melissa M
Kohler, Rainer H
Yang, Katherine S
Laughney, Ashley M
show hidden authors show all authors [1 - 14]
Published in Science Translational Medicine. 2015, vol. 7, no. 314, 314ra183
Abstract Therapeutic nanoparticles (TNPs) have shown heterogeneous responses in human clinical trials, raising questions of whether imaging should be used to identify patients with a higher likelihood of NP accumulation and thus therapeutic response. Despite extensive debate about the enhanced permeability and retention (EPR) effect in tumors, it is increasingly clear that EPR is extremely variable; yet, little experimental data exist to predict the clinical utility of EPR and its influence on TNP efficacy. We hypothesized that a 30-nm magnetic NP (MNP) in clinical use could predict colocalization of TNPs by magnetic resonance imaging (MRI). To this end, we performed single-cell resolution imaging of fluorescently labeled MNPs and TNPs and studied their intratumoral distribution in mice. MNPs circulated in the tumor microvasculature and demonstrated sustained uptake into cells of the tumor microenvironment within minutes. MNPs could predictably demonstrate areas of colocalization for a model TNP, poly(d,l-lactic-co-glycolic acid)-b-polyethylene glycol (PLGA-PEG), within the tumor microenvironment with >85% accuracy and circulating within the microvasculature with >95% accuracy, despite their markedly different sizes and compositions. Computational analysis of NP transport enabled predictive modeling of TNP distribution based on imaging data and identified key parameters governing intratumoral NP accumulation and macrophage uptake. Finally, MRI accurately predicted initial treatment response and drug accumulation in a preclinical efficacy study using a paclitaxel-encapsulated NP in tumor-bearing mice. These approaches yield valuable insight into the in vivo kinetics of NP distribution and suggest that clinically relevant imaging modalities and agents can be used to select patients with high EPR for treatment with TNPs.
Keywords AnimalsAntineoplastic AgentsPhytogenic/administration & dosage/chemistry/metabolismCell LineTumorChemistryPharmaceuticalDNA DamageDisease ProgressionFemaleFerrosoferric Oxide/chemistry/metabolismFibrosarcoma/drug therapy/genetics/metabolism/pathologyHumansMacrophages/metabolismMagnetic Resonance Imaging/methodsMagnetics/methodsMiceInbred BALB CMiceInbred C57BLMiceNudeMiceTransgenicNanomedicine/methodsNanoparticlesOvarian Neoplasms/drug therapy/genetics/metabolism/pathologyPaclitaxel/administration & dosage/chemistry/metabolismParticle SizePolyethylene Glycols/chemistry/metabolismPolyglactin 910/chemistry/metabolismPredictive Value of TestsTime FactorsTissue DistributionTumor MicroenvironmentXenograft Model Antitumor Assays
Identifiers
PMID: 26582898
Full text
Research group Comprendre et manipuler le système immunitaire pour lutter contre le cancer (1024)
Citation
(ISO format)
MILLER, Miles A et al. Predicting therapeutic nanomedicine efficacy using a companion magnetic resonance imaging nanoparticle. In: Science Translational Medicine, 2015, vol. 7, n° 314, p. 314ra183. doi: 10.1126/scitranslmed.aac6522 https://archive-ouverte.unige.ch/unige:154070

60 hits

0 download

Update

Deposited on : 2021-08-24

Export document
Format :
Citation style :