en
Scientific article
Open access
English

Durable and controlled depletion of neutrophils in mice

Published inNature Communications, vol. 11, no. 1, 2762
Publication date2020
Abstract

Neutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies. However, these approaches are only partially effective, transient or lack specificity. Here we report that neutrophils remaining after anti-Ly6G treatment are newly derived from the bone marrow, instead of depletion escapees. Mechanistically, newly generated, circulating neutrophils have lower Ly6G membrane expression, and consequently reduced targets for anti-Ly6G-mediated depletion. To overcome this limitation, we develop a double antibody-based depletion strategy that enhances neutrophil elimination by anti-Ly6G treatment. This approach achieves specific, durable and controlled reduction of neutrophils in vivo, and may be suitable for studying neutrophil function in experimental models.

Keywords
  • Animals
  • Antibodies
  • Monoclonal/metabolism/therapeutic use
  • Antigens
  • Ly/genetics/immunology
  • Bone Marrow/immunology
  • Cell Death
  • Disease Models
  • Animal
  • Gene Expression
  • Immunity
  • Innate
  • Mice
  • Mice
  • Inbred C57BL
  • Neutrophils/immunology
  • RNA
  • Messenger/metabolism
Affiliation Not a UNIGE publication
Citation (ISO format)
BOIVIN, Gael et al. Durable and controlled depletion of neutrophils in mice. In: Nature Communications, 2020, vol. 11, n° 1, p. 2762. doi: 10.1038/s41467-020-16596-9
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal2041-1723
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58downloads

Technical informations

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