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Title

Persistence of self-reactive CD8+ T cells in the CNS requires TOX-dependent chromatin remodeling

Authors
Kaye, Jonathan
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Published in Nature communications. 2021, vol. 12, no. 1, 1009
Abstract Self-reactive CD8+ T cells are important mediators of progressive tissue damage in autoimmune diseases, but the molecular program underlying these cells' functional adaptation is unclear. Here we characterize the transcriptional and epigenetic landscape of self-reactive CD8+ T cells in a mouse model of protracted central nervous system (CNS) autoimmunity and compare it to populations of CNS-resident memory CD8+ T cells emerging from acute viral infection. We find that autoimmune CD8+ T cells persisting at sites of self-antigen exhibit characteristic transcriptional regulation together with distinct epigenetic remodeling. This self-reactive CD8+ T cell fate depends on the transcriptional regulation by the DNA-binding HMG-box protein TOX which remodels more than 400 genomic regions including loci such as Tcf7, which is central to stemness of CD8+ T cells. Continuous exposure to CNS self-antigen sustains TOX levels in self-reactive CD8+ T cells, whereas genetic ablation of TOX in CD8+ T cells results in shortened persistence of self-reactive CD8+ T cells in the inflamed CNS. Our study establishes and characterizes the genetic differentiation program enabling chronic T cell-driven immunopathology in CNS autoimmunity.
Keywords AnimalsAutoimmunity/immunologyCD8-Positive T-Lymphocytes/immunologyCentral Nervous System/metabolismChromatin Assembly and DisassemblyFemaleGene Expression RegulationImmunologic MemoryMaleMiceInbred C57BLKnockout
Identifiers
PMID: 33579927
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Dataset: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE149643
Structures
Research group La Sclérose en plaques (908)
Project
Swiss National Science Foundation: 310030_173010
Citation
(ISO format)
PAGE, Nicolas et al. Persistence of self-reactive CD8+ T cells in the CNS requires TOX-dependent chromatin remodeling. In: Nature Communications, 2021, vol. 12, n° 1, p. 1009. doi: 10.1038/s41467-021-21109-3 https://archive-ouverte.unige.ch/unige:151780

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Deposited on : 2021-05-19

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