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Methods to investigate miRNA function: focus on platelet reactivity

Published in Thrombosis and haemostasis. 2020, vol. 121, no. 4, p. 409-421
Abstract MicroRNAs (miRNAs) are small noncoding RNAs modulating protein production. They are key players in regulation of cell function and are considered as biomarkers in several diseases. The identification of the proteins they regulate, and their impact on cell physiology, may delineate their role as diagnostic or prognostic markers and identify new therapeutic strategies. During the last 3 decades, development of a large panel of techniques has given rise to multiple models dedicated to the study of miRNAs. Since plasma samples are easily accessible, circulating miRNAs can be studied in clinical trials. To quantify miRNAs in numerous plasma samples, the choice of extraction and purification techniques, as well as normalization procedures, are important for comparisons of miRNA levels in populations and over time. Recent advances in bioinformatics provide tools to identify putative miRNAs targets that can then be validated with dedicated assays. In vitro and in vivo approaches aim to functionally validate candidate miRNAs from correlations and to understand their impact on cellular processes. This review describes the advantages and pitfalls of the available techniques for translational research to study miRNAs with a focus on their role in regulating platelet reactivity.
PMID: 33124028
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Research groups Geneva Platelet Group (13)
Recherche translationnelle : anomalies de l'hémostase et thromboses (504)
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GARCIA, Alix et al. Methods to investigate miRNA function: focus on platelet reactivity. In: Thrombosis and Haemostasis, 2020, vol. 121, n° 4, p. 409-421. doi: 10.1055/s-0040-1718730 https://archive-ouverte.unige.ch/unige:150555

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Deposited on : 2021-03-22

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