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Title

Isolation, culture, and genetic engineering of mammalian primary pigment epithelial cells for non-viral gene therapy

Authors
Johnen, Sandra
Izsvák, Zsuzsanna
Published in Journal of Visualized Experiments. 2021, no. 168, p. e62145
Abstract Age-related macular degeneration (AMD) is the most frequent cause of blindness in patients >60 years, affecting ~30 million people worldwide. AMD is a multifactorial disease influenced by environmental and genetic factors, which lead to functional impairment of the retina due to retinal pigment epithelial (RPE) cell degeneration followed by photoreceptor degradation. An ideal treatment would include the transplantation of healthy RPE cells secreting neuroprotective factors to prevent RPE cell death and photoreceptor degeneration. Due to the functional and genetic similarities and the possibility of a less invasive biopsy, the transplantation of iris pigment epithelial (IPE) cells was proposed as a substitute for the degenerated RPE. Secretion of neuroprotective factors by a low number of subretinally-transplanted cells reduce oxidative stress and offers a flexible system for ex vivo analyses and in vivo studies transferable to humans to develop ocular gene therapy approaches.
Identifiers
PMID: 33720134
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Research group Ophtalmologie expérimentale (925)
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BASCUAS CASTILLO, Thaïs et al. Isolation, culture, and genetic engineering of mammalian primary pigment epithelial cells for non-viral gene therapy. In: Journal of Visualized Experiments, 2021, n° 168, p. e62145. doi: 10.3791/62145 https://archive-ouverte.unige.ch/unige:150464

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Deposited on : 2021-03-17

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