Isolation, culture, and genetic engineering of mammalian primary pigment epithelial cells for non-viral gene therapy

Article Article scientifique Article Isolation, culture, and genetic engineering of mammalian primary pigment epithelial cells for non-viral gene therapy eng 2021 author Bascuas Castillo Thaïs 0000-0002-3011-2201 893012 author Kropp Martina 0000-0001-6861-5851 793095 author Harmening Nina 794769 author Wong Brittany 908754 author Johnen Sandra author Izsvák Zsuzsanna author Thumann Gabriele 0000-0003-3682-9569 785873 e62145 10.3791/62145 33720134 1940-087X https://www.jove.com/v/62145/isolation-culture-genetic-engineering-mammalian-primary-pigment Age-related macular degeneration (AMD) is the most frequent cause of blindness in patients >60 years, affecting ~30 million people worldwide. AMD is a multifactorial disease influenced by environmental and genetic factors, which lead to functional impairment of the retina due to retinal pigment epithelial (RPE) cell degeneration followed by photoreceptor degradation. An ideal treatment would include the transplantation of healthy RPE cells secreting neuroprotective factors to prevent RPE cell death and photoreceptor degeneration. Due to the functional and genetic similarities and the possibility of a less invasive biopsy, the transplantation of iris pigment epithelial (IPE) cells was proposed as a substitute for the degenerated RPE. Secretion of neuroprotective factors by a low number of subretinally-transplanted cells reduce oxidative stress and offers a flexible system for ex vivo analyses and in vivo studies transferable to humans to develop ocular gene therapy approaches. Dewey 616.8 61 Département des neurosciences cliniques 226 false 582 Ophtalmologie expérimentale 925 http://jove.com/video/62145 Journal of Visualized Experiments 168 55ce5cf1-6ee8-4f49-bc75-807f29f0a3a1 Article (Published version) Restricted jove-protocol-62145-isolation-culture-genetic-engineering-mammalian-primary-pigment-1.pdf 1909747 application/pdf e813bce3-b468-4a62-b31b-b4ef2b15267d Appendix Restricted jove-materials-62145-isolation-culture-genetic-engineering-mammalian-primary-pigment-1.pdf 77192 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département des neurosciences cliniques

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Isolation, culture, and genetic engineering of mammalian primary pigment epithelial cells for non-viral gene therapy	Journal of Visualized Experiments			2021	443	11

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Wong, Brittany