Scientific article
Open access

Simian virus 40 large tumor antigen: a "RNA binding protein"?

Published inProceedings of the National Academy of Sciences, vol. 79, no. 4, p. 1139-1143
Publication date1982

Simian virus 40 large tumor antigen was isolated by immunoaffinity chromatography from monkey or mouse cell cultures undergoing lytic or transforming infection. RNase-treated gel-purified large tumor antigen, on hydrolysis with alkali, gave about equimolar amounts of AMP, GMP, CMP, and UMP. Furthermore, RNA fragments of approximately 45 nucleotides could be isolated from large tumor antigen purified by the same procedure. Mapping of the T1 oligonucleotides showed a high complexity, as indicated by the presence of unique sequences of 15-30 nucleotides and of poly(A). This is compatible with the hypothesis that these RNA fragments are derived from cellular pre-mRNAs or mRNAs. Our results suggest that Simian virus 40 large tumor antigen is a RNA-binding protein and might possibly be involved in regulation of synthesis, maturation, or translation of cellular mRNAs.

  • Electrofocusing
  • Messenger ribonucleoprotein
  • Phosphoprotein
  • Regulation of transcription and translation
  • Swiss National Science Foundation - 3.474.79
  • Swiss National Science Foundation - 3.664.80
Citation (ISO format)
KHANDJIAN, Edouard W. et al. Simian virus 40 large tumor antigen: a ‘RNA binding protein’? In: Proceedings of the National Academy of Sciences, 1982, vol. 79, n° 4, p. 1139–1143. doi: 10.1073/pnas.79.4.1139
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Article (Published version)
ISSN of the journal0027-8424

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