Simian virus 40 large tumor antigen: a "RNA binding protein"?

Article Article scientifique Article Simian virus 40 large tumor antigen: a "RNA binding protein"? eng 1982 author Khandjian Edouard W. author Loche Michel 26413 author Darlix Jean-Luc 63109 author Cramer R. author Turler Hans 25123 author Weil Roger 25112 1139-1143 10.1073/pnas.79.4.1139 0027-8424 http://www.pnas.org/cgi/doi/10.1073/pnas.79.4.1139 Simian virus 40 large tumor antigen was isolated by immunoaffinity chromatography from monkey or mouse cell cultures undergoing lytic or transforming infection. RNase-treated gel-purified large tumor antigen, on hydrolysis with alkali, gave about equimolar amounts of AMP, GMP, CMP, and UMP. Furthermore, RNA fragments of approximately 45 nucleotides could be isolated from large tumor antigen purified by the same procedure. Mapping of the T1 oligonucleotides showed a high complexity, as indicated by the presence of unique sequences of 15-30 nucleotides and of poly(A). This is compatible with the hypothesis that these RNA fragments are derived from cellular pre-mRNAs or mRNAs. Our results suggest that Simian virus 40 large tumor antigen is a RNA-binding protein and might possibly be involved in regulation of synthesis, maturation, or translation of cellular mRNAs. Electrofocusing Messenger ribonucleoprotein Phosphoprotein Regulation of transcription and translation Dewey 570 Swiss National Science Foundation 3.474.79 CH Swiss National Science Foundation 3.664.80 CH 43 Département de biologie moléculaire 161 false Proceedings of the National Academy of Sciences 79 4 1f33ab02-8f9d-44ff-b7ae-f3ff19ebd150 Article (Published version) Public 1139.full.pdf 1774365 application/pdf CC BY openAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Simian virus 40 large tumor antigen: a "RNA binding protein"?	Proceedings of the National Academy of Sciences			1982	217	196

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Loche, Michel