en
Scientific article
English

Following electroenzymatic hydrogen production by rotating ring disk electrochemistry and mass spectrometry

Published inAngewandte Chemie: International Edition, vol. 60, no. 18, p. 10001-10006
Publication date2021
Abstract

Gas‐processing metalloenzymes are of interest to future bio‐ and bioinspired technologies. Of particular importance are hydrogenases and nitrogenases, which both produce molecular hydrogen (H2) from proton (H+) reduction. Here, we report on the use of rotating ring disk electrochemistry (RRDE) and mass spectrometry (MS) to follow the production of H2 and isotopes produced from deuteron (D+) reduction (HD and D2) using the [FeFe]‐hydrogenase from Clostridium pasteurianum, a model hydrogen‐evolving metalloenzyme. This facilitates enzymology studies independent of non‐innocent chemical reductants. We anticipate that these approaches will be of value in resolving the catalytic mechanisms of H2 ‐producing metalloenzymes and the design of bioinspired catalysts for H2 production and N2 fixation.

Keywords
  • Enzymatic electrochemistry
  • [FeFe]
  • Hydrogen
  • Kinetic isotope effect
  • Metalloenzymes
Citation (ISO format)
KHUSHVAKOV, Jaloliddin et al. Following electroenzymatic hydrogen production by rotating ring disk electrochemistry and mass spectrometry. In: Angewandte Chemie: International Edition, 2021, vol. 60, n° 18, p. 10001–10006. doi: 10.1002/anie.202100863
Main files (1)
Article (Submitted version)
accessLevelPublic
Identifiers
ISSN of the journal1433-7851
269views
224downloads

Technical informations

Creation02/25/2021 5:28:00 PM
First validation02/25/2021 5:28:00 PM
Update time03/16/2023 12:09:24 AM
Status update03/16/2023 12:09:23 AM
Last indexation05/06/2024 6:44:20 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack