Following electroenzymatic hydrogen production by rotating ring disk electrochemistry and mass spectrometry

Article Article scientifique Article Following electroenzymatic hydrogen production by rotating ring disk electrochemistry and mass spectrometry eng 2021 author Khushvakov Jaloliddin 923343 author Nussbaum Robin 0000-0001-7311-4023 856780 author Cadoux Cécile 932096 author Duan Jifu author Stripp Sven author Milton Ross Dean 0000-0002-2229-0243 930839 10001-10006 10.1002/anie.202100863 1433-7851 https://onlinelibrary.wiley.com/doi/10.1002/anie.202100863 Gas‐processing metalloenzymes are of interest to future bio‐ and bioinspired technologies. Of particular importance are hydrogenases and nitrogenases, which both produce molecular hydrogen (H2) from proton (H+) reduction. Here, we report on the use of rotating ring disk electrochemistry (RRDE) and mass spectrometry (MS) to follow the production of H2 and isotopes produced from deuteron (D+) reduction (HD and D2) using the [FeFe]‐hydrogenase from Clostridium pasteurianum, a model hydrogen‐evolving metalloenzyme. This facilitates enzymology studies independent of non‐innocent chemical reductants. We anticipate that these approaches will be of value in resolving the catalytic mechanisms of H2 ‐producing metalloenzymes and the design of bioinspired catalysts for H2 production and N2 fixation. Enzymatic electrochemistry [FeFe] Hydrogen Kinetic isotope effect Metalloenzymes Dewey 540 40 Département de chimie minérale, analytique et appliquée 145 false Angewandte Chemie: International Edition 60 18 735e3f2d-0514-41a7-a036-4357020ef973 Article (Submitted version) Public RRDE_MS_Self_Archive.pdf 1286223 application/pdf openAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie minérale, analytique et appliquée

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Following electroenzymatic hydrogen production by rotating ring disk electrochemistry and mass spectrometry	Angewandte Chemie: International Edition			2021	328	441

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Khushvakov, Jaloliddin