en
Scientific article
English

Microcomedones in non‐lesional acne prone skin New orientations on comedogenesis and its prevention

Publication date2020
Abstract

Background In non-lesional skin of acne patients, cyanoacrylate skin surface stripping can harvest a structure called microcomedone (MC) which is the earliest phase of comedogenesis; the root of any subsequent clinical lesion and a target for the prevention of acne relapses. More information is needed on the putative biochemical contributors (biomarkers) of comedogenesis expressed in MC. Methods Proteins expressed in MC were screened by proteomics, immunohistochemistry and Western blotting. The in vitro effects of a comedolytic Silybum marianum fruit extract (SMFE) were studied in sebocyte cultures by RNA-Seq and modulation of CYP1A1 by qPCR and enzymatic activity. MC severity was correlated to lesions counts and keratin expression during 48 weeks in 23 acne patients using a topical comedolytic formulation containing SMFE. Results Two infundibular keratins, K75 and K79, co-localized in MC with the sebocyte progenitor cell marker LRIG1 and were used as a biomarker of comedogenesis for the follow-up of patients. In cultured sebocytes exposed to SMFE (i) transcriptomic analysis showed an up-regulation by a factor of 15 of RNA coding for K75 and (ii) the gene expression and catalytic activity of CYP1A1 under exposure to dioxin was decreased. In the acne patients using SMFE, the MC index in nonlesional skin decreased over time and remained until the 48th week, significantly lower than that of the first week. There was a high correlation between the decrease of MC index and the decrease and stability of the clinical lesions counts over time. Importantly, a low MC index status was found to be associated with a significant higher K75 expression in microcomedones. Discussion These observations provide new orientations on the mechanism of comedogenesis and its prevention. Maintaining a low MC status in non-lesional skin is a sound target for the prevention of acne relapse and a good sentinel of acne remissions.

Keywords
  • Acne
  • Microcomedones
  • Skin surface biopsies
  • Treatment
  • Biomarkers
Funding
  • Autre - Fonds UF1024, University of Geneva
Citation (ISO format)
FONTAO, Fabienne et al. Microcomedones in non‐lesional acne prone skin New orientations on comedogenesis and its prevention. In: Journal of the European Academy of Dermatology and Venereology, 2020, vol. 34, n° 2, p. 357–364. doi: 10.1111/jdv.15926
Main files (1)
Article (Published version)
accessLevelRestricted
Secondary files (2)
Identifiers
ISSN of the journal0926-9959
177views
1downloads

Technical informations

Creation10/28/2020 10:52:00 AM
First validation10/28/2020 10:52:00 AM
Update time03/16/2023 12:00:53 AM
Status update03/16/2023 12:00:51 AM
Last indexation01/17/2024 12:23:44 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack