Microcomedones in non‐lesional acne prone skin New orientations on comedogenesis and its prevention

Article Article scientifique Article Microcomedones in non‐lesional acne prone skin New orientations on comedogenesis and its prevention eng 2020 author Fontao Fabienne 133879 author Von Engelbrechten Martine Dominique 60546 author Seilaz Catherine 134014 author Sorg Olivier 32172 author Saurat Jean-Hilaire 21995 357-364 10.1111/jdv.15926 31465602 0926-9959 https://onlinelibrary.wiley.com/doi/abs/10.1111/jdv.15926 Background In non-lesional skin of acne patients, cyanoacrylate skin surface stripping can harvest a structure called microcomedone (MC) which is the earliest phase of comedogenesis; the root of any subsequent clinical lesion and a target for the prevention of acne relapses. More information is needed on the putative biochemical contributors (biomarkers) of comedogenesis expressed in MC. Methods Proteins expressed in MC were screened by proteomics, immunohistochemistry and Western blotting. The in vitro effects of a comedolytic Silybum marianum fruit extract (SMFE) were studied in sebocyte cultures by RNA-Seq and modulation of CYP1A1 by qPCR and enzymatic activity. MC severity was correlated to lesions counts and keratin expression during 48 weeks in 23 acne patients using a topical comedolytic formulation containing SMFE. Results Two infundibular keratins, K75 and K79, co-localized in MC with the sebocyte progenitor cell marker LRIG1 and were used as a biomarker of comedogenesis for the follow-up of patients. In cultured sebocytes exposed to SMFE (i) transcriptomic analysis showed an up-regulation by a factor of 15 of RNA coding for K75 and (ii) the gene expression and catalytic activity of CYP1A1 under exposure to dioxin was decreased. In the acne patients using SMFE, the MC index in nonlesional skin decreased over time and remained until the 48th week, significantly lower than that of the first week. There was a high correlation between the decrease of MC index and the decrease and stability of the clinical lesions counts over time. Importantly, a low MC index status was found to be associated with a significant higher K75 expression in microcomedones. Discussion These observations provide new orientations on the mechanism of comedogenesis and its prevention. Maintaining a low MC status in non-lesional skin is a sound target for the prevention of acne relapse and a good sentinel of acne remissions. Acne Microcomedones Skin surface biopsies Treatment Biomarkers Dewey 617 Autre Fonds UF1024, University of Geneva 63 Département d'anesthésiologie, pharmacologie, soins intensifs et urgences 229 false 386 La recherche en biochimie dermatologique 652 Journal of the European Academy of Dermatology and Venereology 34 2 8178a889-3807-4021-97ec-c83a017520ec Article (Published version) Restricted Acné_Patients.pdf 1013655 application/pdf 60bdd679-a52a-4795-8790-e86ab44d4540 Appendix Restricted jdv15926-sup-0001-supinfo.pdf 206681 application/pdf ae609887-f41a-4979-800b-e1379dcbdc53 Supplemental data Restricted jdv15926-sup-0002-figures1-s2.pdf 146988 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département d'anesthésiologie, pharmacologie, soins intensifs et urgences

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Microcomedones in non‐lesional acne prone skin New orientations on comedogenesis and its prevention	Journal of the European Academy of Dermatology and Venereology			2020	348	1

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Seilaz, Catherine