Development of an in-capillary approach to nanoscale automated in vitro cytochromes p450 assays

Nicoli, Raul; Curcio, Raffaele; Rudaz, Serge; Veuthey, Jean-Luc

doi:10.1021/jm900201b

Scientific article

English

Development of an in-capillary approach to nanoscale automated in vitro cytochromes p450 assays

Contributors

Nicoli, Raul; Curcio, Raffaele; Rudaz, Serge

; Veuthey, Jean-Luc

Published in

Journal of medicinal chemistry, vol. 52, no. 8, p. 2192-2195

Publication date

2009

Abstract

A method to perform nanoscale automated CYP450-based drug metabolism studies using a capillary as a reaction vessel is described. In-capillary assays consumed only approximately 30 nL of recombinant human CYP450 solution. Ultrafast analysis of substrates and metabolites was achieved off-line by ultrahigh-pressure liquid chromatography coupled to mass spectrometry. This approach was successfully applied to qualitative metabolism studies of six major CYP450 isozymes and CYP2D6 inhibition experiments.

UNIGE affiliation entities

Faculté des sciences / Section des sciences pharmaceutiques

Citation (ISO format)

NICOLI, Raul et al. Development of an in-capillary approach to nanoscale automated in vitro cytochromes p450 assays. In: Journal of medicinal chemistry, 2009, vol. 52, n° 8, p. 2192–2195. doi: 10.1021/jm900201b

Article (Accepted version)

Identifiers

PID : unige:1471
DOI : 10.1021/jm900201b
PMID : 19326942

Journal ISSN

0022-2623

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24/04/2009 11:58:00

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Development of an in-capillary approach to nanoscale automated in vitro cytochromes p450 assays

Contributors

Published in

Publication date

Abstract

UNIGE affiliation entities

Citation (ISO format)

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Technical informations

Creation

First validation

Update

Status update

Last indexation