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Development of an in-capillary approach to nanoscale automated in vitro cytochromes p450 assays

Published in Journal of medicinal chemistry. 2009, vol. 52, no. 8, p. 2192-2195
Abstract A method to perform nanoscale automated CYP450-based drug metabolism studies using a capillary as a reaction vessel is described. In-capillary assays consumed only approximately 30 nL of recombinant human CYP450 solution. Ultrafast analysis of substrates and metabolites was achieved off-line by ultrahigh-pressure liquid chromatography coupled to mass spectrometry. This approach was successfully applied to qualitative metabolism studies of six major CYP450 isozymes and CYP2D6 inhibition experiments.
Stable URL https://archive-ouverte.unige.ch/unige:1471
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Other version: http://pubs.acs.org/doi/pdfplus/10.1021/jm900201b
PMID: 19326942

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Deposited on : 2009-05-04

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