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Quality control of pharmaceutical formulations containing cisplatin, carboplatin, and oxaliplatin by micellar and microemulsion electrokinetic chromatography (MEKC, MEEKC)

Fleury-Souverain, Sandrine
Published in Journal of pharmaceutical and biomedical analysis. 2011, vol. 55, no. 2, p. 253-258
Abstract A micellar electrokinetic chromatography (MEKC) method was developed for the determination of cisplatin, carboplatin, and oxaliplatin in pharmaceutical formulations. The background electrolyte consisted of a phosphate buffer (pH 7.0; 25mM) with sodium dodecyl sulfate (80mM). The applied voltage was 30kV and the sample injection was performed in the hydrodynamic mode. All analyses were carried out in a fused silica capillary with an internal diameter of 50μm and a total length of 64.5cm. The detection of target compounds was performed at 200nm. Under these conditions, a complete separation of cisplatin, carboplatin and oxaliplatin was achieved in less than 10min. The MEKC-UV method was validated and trueness values between 99.7% and 100.8% were obtained with repeatability and intermediate precision values of 0.7-1.4% and 1.1-1.7%, respectively for the three drugs. This method was found appropriate for controlling pharmaceutical formulations containing platinum complexes and successfully applied in quality control at the Geneva University Hospitals.
Keywords Anticancer drugsCapillary electrophoresisPlatinum drugsMicellar electrokinetic chromatography (MEKC)Quality controlValidation
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PMID: 21330090

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Deposited on : 2011-03-10

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