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Title

Optimization by infusion of multiple reaction monitoring transitions for sensitive quantification of peptides by liquid chromatography/mass spectrometry
Authors
Alghanem, Bandar
Nikitin, Frédéric
Stricker, Thomas
Duchoslav, Eva
Luban, Jeremy
Strambio-De-Castillia, Caterina
Muller, Markus Johann
Lisacek, Frédérique
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Published in Rapid Communications in Mass Spectrometry. 2017, vol. 31, no. 9, p. 753-761
Abstract In peptide quantification by liquid chromatography/mass spectrometry (LC/MS), the optimization of multiple reaction monitoring (MRM) parameters is essential for sensitive detection. We have compared different approaches to build MRM assays, based either on flow injection analysis (FIA) of isotopically labelled peptides, or on the knowledge and the prediction of the best settings for MRM transitions and collision energies (CE). In this context, we introduce MRMOptimizer, an open‐source software tool that processes spectra and assists the user in selecting transitions in the FIA workflow.
Identifiers
PMID: 28199054
Full text
Article (Published version) (763 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://doi.wiley.com/10.1002/rcm.7839
Structures
Faculté des sciences / Section des sciences pharmaceutiques
Faculté des sciences / Section de chimie et biochimie / Département de chimie minérale, analytique et appliquée
Citation
(ISO format) 		ALGHANEM, Bandar et al. Optimization by infusion of multiple reaction monitoring transitions for sensitive quantification of peptides by liquid chromatography/mass spectrometry. In: Rapid Communications in Mass Spectrometry, 2017, vol. 31, n° 9, p. 753-761. doi: 10.1002/rcm.7839 https://archive-ouverte.unige.ch/unige:144951

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Deposited on : 2020-11-19

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