UNIGE document Scientific Article
previous document  unige:144951  next document
add to browser collection
Title

Optimization by infusion of multiple reaction monitoring transitions for sensitive quantification of peptides by liquid chromatography/mass spectrometry

Authors
Duchoslav, Eva
Strambio-De-Castillia, Caterina
show hidden authors show all authors [1 - 10]
Published in Rapid Communications in Mass Spectrometry. 2017, vol. 31, no. 9, p. 753-761
Abstract In peptide quantification by liquid chromatography/mass spectrometry (LC/MS), the optimization of multiple reaction monitoring (MRM) parameters is essential for sensitive detection. We have compared different approaches to build MRM assays, based either on flow injection analysis (FIA) of isotopically labelled peptides, or on the knowledge and the prediction of the best settings for MRM transitions and collision energies (CE). In this context, we introduce MRMOptimizer, an open‐source software tool that processes spectra and assists the user in selecting transitions in the FIA workflow.
Identifiers
PMID: 28199054
Full text
Article (Published version) (763 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://doi.wiley.com/10.1002/rcm.7839
Structures
Citation
(ISO format)
ALGHANEM, Bandar et al. Optimization by infusion of multiple reaction monitoring transitions for sensitive quantification of peptides by liquid chromatography/mass spectrometry. In: Rapid Communications in Mass Spectrometry, 2017, vol. 31, n° 9, p. 753-761. doi: 10.1002/rcm.7839 https://archive-ouverte.unige.ch/unige:144951

24 hits

0 download

Update

Deposited on : 2020-11-19

Export document
Format :
Citation style :