en
Scientific article
English

Mutational analysis of the phage T4 morphogenetic 31 gene, whose product interacts with the Escherichia coli GroEL protein

Published inGene, vol. 86, no. 1, p. 19-25
Publication date1990
Abstract

The phage T4 morphogenetic gene 31 has been sequenced. Its deduced gene product is a polypeptide of 111 aa, with a predicted Mr of 12064 and a pI of 4.88. The proof that the assigned open reading frame (ORF) encodes Gp31 rests on the sequencing of two known gene 31 amber mutations, amN54 and NG71, demonstrating that these mutations result in translational termination within the assigned ORF. Furthermore, the sequencing of four different T4 epsilon mutations, isolated on the basis of allowing the phage to propagate on Escherichia coli groEL- hosts, showed that they are either missense mutations or 3-bp deletions in the gene 31 reading frame. The sequencing of neighboring DNA revealed the presence of five other ORFs, one of which overlaps gene 31 substantially, but in the opposite orientation.

Keywords
  • Amino acid sequence
  • Bacterial proteins/metabolism
  • Base sequence
  • Molecular cloning
  • DNA mutational analysis
  • Viral genes
  • Molecular sequence data
  • Morphogenesis
  • T-Phages/genetics/growth & development
  • Viral proteins/genetics
  • Viral structural proteins/genetics
  • Virus replication
  • Recombinant DNA
  • Nucleotide sequence
  • Amber mutations
  • Phage head assembly
  • Heat shock proteins
  • Protein-protein interactions
Funding
  • Swiss National Science Foundation - 3.231
Citation (ISO format)
KEPPEL, France et al. Mutational analysis of the phage T4 morphogenetic <i>31</i> gene, whose product interacts with the <i>Escherichia coli</i> GroEL protein. In: Gene, 1990, vol. 86, n° 1, p. 19–25. doi: 10.1016/0378-1119(90)90109-5
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Article (Published version)
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Identifiers
ISSN of the journal0378-1119
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