Mutational analysis of the phage T4 morphogenetic 31 gene, whose product interacts with the Escherichia coli GroEL protein

Article Article scientifique Article Mutational analysis of the phage T4 morphogenetic 31 gene, whose product interacts with the Escherichia coli GroEL protein eng 1990 author Keppel France author Lipinska Barbara author Ang Deborah 27983 author Georgopoulos Costa Panos 27799 19-25 10.1016/0378-1119(90)90109-5 2311934 0378-1119 The phage T4 morphogenetic gene 31 has been sequenced. Its deduced gene product is a polypeptide of 111 aa, with a predicted Mr of 12064 and a pI of 4.88. The proof that the assigned open reading frame (ORF) encodes Gp31 rests on the sequencing of two known gene 31 amber mutations, amN54 and NG71, demonstrating that these mutations result in translational termination within the assigned ORF. Furthermore, the sequencing of four different T4 epsilon mutations, isolated on the basis of allowing the phage to propagate on Escherichia coli groEL- hosts, showed that they are either missense mutations or 3-bp deletions in the gene 31 reading frame. The sequencing of neighboring DNA revealed the presence of five other ORFs, one of which overlaps gene 31 substantially, but in the opposite orientation. Amino acid sequence Bacterial proteins/metabolism Base sequence Molecular cloning DNA mutational analysis Viral genes Molecular sequence data Morphogenesis T-Phages/genetics/growth & development Viral proteins/genetics Viral structural proteins/genetics Virus replication Recombinant DNA Nucleotide sequence Amber mutations Phage head assembly Heat shock proteins Protein-protein interactions Dewey 570 Swiss National Science Foundation 3.231 CH 43 Département de biologie moléculaire 161 false Gene 86 1 7bf18710-1305-4eea-81e6-42787f6c8ffe Article (Published version) Restricted 1-s2.0-0378111990901095-main.pdf 1272103 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Mutational analysis of the phage T4 morphogenetic 31 gene, whose product interacts with the Escherichia coli GroEL protein	Gene			1990	185	0

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Ang, Deborah