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Title

Rapid identification by MALDI-TOF/MS and antimicrobial disk diffusion susceptibility testing for positive blood cultures after a short incubation on the WASPLab

Authors
Schorderet, Didier
Published in European Journal of Clinical Microbiology & Infectious Diseases. 2020, vol. 39, no. 6, p. 1063-1070
Abstract The objectives of this study were to define the shortest incubation times on the WASPLab for reliable MALDI-TOF/MS-based species identification and for the preparation of a 0.5 McFarland suspension for antimicrobial disk diffusion susceptibility testing using short subcultures growing on solid culture media inoculated by positive blood cultures spiked with a wide range of pathogens associated with bloodstream infections. The 520 clinical strains (20 × 26 different species) included in this study were obtained from a collection of non-consecutive and non-duplicate pathogens identified at Geneva University Hospitals. After 4 h of incubation on the WASPLab, microorganisms' growth allowed accurate identification of 73% (380/520) (95% CI, 69.1-76.7%) of the strains included in this study. The identification rate increased to 85% (440/520) (95% CI, 81.3-87.5%) after 6-h incubation. When excluding Corynebacterium and Candida spp., the microbial growth was sufficient to permit accurate identification of all tested species (100%, 460/460) (95% CI, 99.2-100%) after 8-h incubation. With the exception of Burkholderia cepacia and Haemophilus influenzae, AST by disk diffusion could be performed for Enterobacterales and non-fermenting Gram-negative bacilli after only 4 h of growth in the WASPLab. The preparation of a 0.5 McFarland suspension for Gram-positive bacteria required incubation times ranging between 3 and 8 h according to the bacterial species. Only Corynebacterium spp. required incubation times as long as 16 h. The WASPLab enables rapid pathogen identification as well as swift comprehensive AST from positive blood cultures that can be implemented without additional costs nor hands-on time by defining optimal time points for image acquisition.
Keywords AST by disk diffusionAccuracyAutomationCopan WASPLabMALDI-TOF/MSShort subculturesTurn-around time
Identifiers
PMID: 31965365
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Article (Published version) (1.3 MB) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://link.springer.com/10.1007/s10096-020-03817-8
Structures
Research groups Analyse génomique et fonctionnelle du staphylocoque doré (604)
Chimie et protéomique clinique (270)
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(ISO format)
CHERKAOUI, Abdessalam et al. Rapid identification by MALDI-TOF/MS and antimicrobial disk diffusion susceptibility testing for positive blood cultures after a short incubation on the WASPLab. In: European Journal of Clinical Microbiology & Infectious Diseases, 2020, vol. 39, n° 6, p. 1063-1070. doi: 10.1007/s10096-020-03817-8 https://archive-ouverte.unige.ch/unige:140129

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Deposited on : 2020-08-24

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