Book Chapter
previous document  unige:138461  next document
add to browser collection
Title

Detecting and analyzing murine innate lymphoid cells

Authors
Gomez-Cadena, Alejandra
Romero, Pedro
Trabanelli, Sara
Published in Galluzzi, L. & Rudqvist, N.-P. Tumor Immunology and Immunotherapy: Cellular Methods Part A. Cambridge MA: Elsevier. 2020, p. 329-342
Collection Methods in Enzymology; 631
Abstract During the last 10 years, different subsets of Innate lymphoid cells (ILCs) have been identified in murine models. ILCs play and important role in maintaining immune barriers, tissue homeostasis, and are able to regulate the immune response in several anatomic sites. They can be found in lymphoid and non-lymphoid organs of adult mice but are mainly tissue-resident cells that can expand locally under physiologic or pathologic conditions (Gasteiger, Fan, Dikiy, Lee, & Rudensky, 2015). Because ILCs need to be identified by a complex combination of several cell-surface and intracellular markers and by their production of specific sets of cytokines, multiparametric flow cytometry remains one of the most efficient methods to analyze and isolate the different ILC sub-populations. This chapter describes how ILCs can be identified in different murine organs and how ILC subsets can be isolated and functionally analyzed.
Keywords Innate lymphoid cells (ILC)Innate immunityImmune regulation
Identifiers
PMID: 31948555
ISBN: 978-0-12-818673-2
Full text
Research group Ciblage des lymphocytes sécrétant des cytokines (1015)
Citation
(ISO format)
GOMEZ-CADENA, Alejandra et al. Detecting and analyzing murine innate lymphoid cells. In: Galluzzi, L. & Rudqvist, N.-P. (Ed.). Tumor Immunology and Immunotherapy: Cellular Methods Part A. Cambridge MA : Elsevier, 2020. p. 329-342. (Methods in Enzymology; 631) doi: 10.1016/bs.mie.2019.10.022 https://archive-ouverte.unige.ch/unige:138461

51 hits

0 download

Update

Deposited on : 2020-07-16

Export document
Format :
Citation style :