Potential in vivo transfer of a blaCTX-M14-harbouring plasmid established by combining long- and short-read sequencing

Xavier, Basil Britto; Renzi, Gesuele; Lammens, Christine; Cherkaoui, Abdessalam; Goossens, Herman; Schrenzel, Jacques; Harbarth, Stéphan Juergen; Malhotra-Kumar, Surbhi

doi:10.1016/j.mimet.2019.02.004

Scientific article

English

Potential in vivo transfer of a blaCTX-M14-harbouring plasmid established by combining long- and short-read sequencing

ContributorsXavier, Basil Britto; Renzi, Gesuele; Lammens, Christine; Cherkaoui, Abdessalam; Goossens, Herman; Schrenzel, Jacques; Harbarth, Stéphan Juergen

; Malhotra-Kumar, Surbhi

Published inJournal of Microbiological Methods, vol. 159, p. 1-4

Publication date2019

Abstract

Horizontal transfer of plasmid-mediated antibiotic resistance has rarely been documented in vivo. Utilizing long-read (Oxford Nanopore) and short-read (Illumina) sequencing, we confirmed that a gut-colonizing Escherichia coli and a hypervirulent Klebsiella pneumoniae ST23, isolated from a surgical site culture of a patient receiving cefuroxime therapy, harboured a 100% identical blaCTX-M-14-harbouring plasmid, indicative of a potential transfer in vivo.

Keywords

E coli
ESBL
Extended-spectrum beta-lactamases
Horizontal transfer
IncFII plasmid
K. pneumoniae
Nanopore sequencing
ST23

Affiliation entities

Faculté de médecine / Section de médecine clinique / Département de médecine

Research groups

Citation (ISO format)

XAVIER, Basil Britto et al. Potential in vivo transfer of a blaCTX-M14-harbouring plasmid established by combining long- and short-read sequencing. In: Journal of Microbiological Methods, 2019, vol. 159, p. 1–4. doi: 10.1016/j.mimet.2019.02.004

Article (Published version)

Identifiers

PID : unige:132969
DOI : 10.1016/j.mimet.2019.02.004
PMID : 30738818

ISSN of the journal0167-7012

267views

233downloads

Creation10/30/2019 2:55:00 PM

First validation10/30/2019 2:55:00 PM

Update time10/08/2024 9:10:02 AM

Status update10/08/2024 9:10:02 AM

Last indexation10/31/2024 6:06:37 PM

Archive ouverte UNIGE

Potential in vivo transfer of a blaCTX-M14-harbouring plasmid established by combining long- and short-read sequencing

Technical informations