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Smartphone DNA or RNA Sensing Using Semisynthetic Luciferase-Based Logic Device

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Published in ACS Sensors. 2020, vol. 5, no. 3, p. 807-813
Abstract Detection of specific oligonucleotide sequences is central to numerous applications, and technologies amenable to point-of-care diagnostics or end users are needed. Here, we report a technology making use of a bioluminescent readout and smartphone quantification. The sensor is a semisynthetic luciferase (H-Luc-PNA conjugate) that is turned on by a strand-displacement reaction. We demonstrated sensing of three different microRNAs (miRs), as representative cancer biomarkers, and demonstrate the possibility to integrate an AND gate to sense two sequences simultaneously.
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Research group Groupe Winssinger
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CHANG, Dalu et al. Smartphone DNA or RNA Sensing Using Semisynthetic Luciferase-Based Logic Device. In: ACS Sensors, 2020, vol. 5, n° 3, p. 807-813. doi: 10.1021/acssensors.9b02454 https://archive-ouverte.unige.ch/unige:132816

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Deposited on : 2020-03-23

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