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Scientific article
Open access
English

Smartphone DNA or RNA Sensing Using Semisynthetic Luciferase-Based Logic Device

Published inACS Sensors, vol. 5, no. 3, p. 807-813
Publication date2020
Abstract

Detection of specific oligonucleotide sequences is central to numerous applications, and technologies amenable to point-of-care diagnostics or end users are needed. Here, we report a technology making use of a bioluminescent readout and smartphone quantification. The sensor is a semisynthetic luciferase (H-Luc-PNA conjugate) that is turned on by a strand-displacement reaction. We demonstrated sensing of three different microRNAs (miRs), as representative cancer biomarkers, and demonstrate the possibility to integrate an AND gate to sense two sequences simultaneously.

Research group
Citation (ISO format)
CHANG, Dalu et al. Smartphone DNA or RNA Sensing Using Semisynthetic Luciferase-Based Logic Device. In: ACS Sensors, 2020, vol. 5, n° 3, p. 807–813. doi: 10.1021/acssensors.9b02454
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ISSN of the journal2379-3694
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Technical informations

Creation03/18/2020 5:23:00 PM
First validation03/18/2020 5:23:00 PM
Update time03/15/2023 9:18:46 PM
Status update03/15/2023 9:18:46 PM
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