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Rescue of an aggressive female sexual courtship in mice by CRISPR/Cas9 secondary mutation in vivo

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Published in BMC Research Notes. 2018, vol. 11, no. 1, 193
Abstract We had previously reported a mouse line carrying the Atypical female courtship (HoxD Afc ) allele, where an ectopic accumulation of Hoxd10 transcripts was observed in a sparse population of cells in the adult isocortex, as a result of a partial deletion of the HoxD gene cluster. Female mice carrying this allele displayed an exacerbated paracopulatory behavior, culminating in a severe mutilation of the studs' external genitals. To unequivocally demonstrate that this intriguing phenotype was indeed caused by an illegitimate function of the HOXD10 protein, we use CRISPR/Cas9 technology to induce a microdeletion into the homeobox of the Hoxd10 gene in cis with the HoxD Afc allele.
Keywords AnimalsCRISPR-Cas SystemsCholecystokinin/geneticsCourtshipFemaleGene ExpressionGlutamate Decarboxylase/geneticsHomeodomain Proteins/geneticsIn Situ HybridizationFluorescenceMaleMiceMutationNeocortex/cytology/metabolism/physiologySequence DeletionTranscription Factors/genetics
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PMID: 29580290
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ZAKANY, Jozsef, DUBOULE, Denis. Rescue of an aggressive female sexual courtship in mice by CRISPR/Cas9 secondary mutation in vivo. In: BMC Research Notes, 2018, vol. 11, n° 1, p. 193. doi: 10.1186/s13104-018-3307-8 https://archive-ouverte.unige.ch/unige:131210

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Deposited on : 2020-02-24

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