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Scientific article
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Rescue of an aggressive female sexual courtship in mice by CRISPR/Cas9 secondary mutation in vivo

Published inBMC Research Notes, vol. 11, no. 1, 193
Publication date2018
Abstract

We had previously reported a mouse line carrying the Atypical female courtship (HoxD Afc ) allele, where an ectopic accumulation of Hoxd10 transcripts was observed in a sparse population of cells in the adult isocortex, as a result of a partial deletion of the HoxD gene cluster. Female mice carrying this allele displayed an exacerbated paracopulatory behavior, culminating in a severe mutilation of the studs' external genitals. To unequivocally demonstrate that this intriguing phenotype was indeed caused by an illegitimate function of the HOXD10 protein, we use CRISPR/Cas9 technology to induce a microdeletion into the homeobox of the Hoxd10 gene in cis with the HoxD Afc allele.

Keywords
  • Animals
  • CRISPR-Cas Systems
  • Cholecystokinin/genetics
  • Courtship
  • Female
  • Gene Expression
  • Glutamate Decarboxylase/genetics
  • Homeodomain Proteins/genetics
  • In Situ Hybridization
  • Fluorescence
  • Male
  • Mice
  • Mutation
  • Neocortex/cytology/metabolism/physiology
  • Sequence Deletion
  • Transcription Factors/genetics
Citation (ISO format)
ZAKANY, Jozsef, DUBOULE, Denis. Rescue of an aggressive female sexual courtship in mice by CRISPR/Cas9 secondary mutation in vivo. In: BMC Research Notes, 2018, vol. 11, n° 1, p. 193. doi: 10.1186/s13104-018-3307-8
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Article (Published version)
Identifiers
ISSN of the journal1756-0500
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