Rescue of an aggressive female sexual courtship in mice by CRISPR/Cas9 secondary mutation in vivo

Zakany, Jozsef; Duboule, Denis

doi:10.1186/s13104-018-3307-8

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Title		Rescue of an aggressive female sexual courtship in mice by CRISPR/Cas9 secondary mutation in vivo
Authors		Zakany, Jozsef Duboule, Denis
Published in		BMC Research Notes. 2018, vol. 11, no. 1, 193
Abstract		We had previously reported a mouse line carrying the Atypical female courtship (HoxD Afc ) allele, where an ectopic accumulation of Hoxd10 transcripts was observed in a sparse population of cells in the adult isocortex, as a result of a partial deletion of the HoxD gene cluster. Female mice carrying this allele displayed an exacerbated paracopulatory behavior, culminating in a severe mutilation of the studs' external genitals. To unequivocally demonstrate that this intriguing phenotype was indeed caused by an illegitimate function of the HOXD10 protein, we use CRISPR/Cas9 technology to induce a microdeletion into the homeobox of the Hoxd10 gene in cis with the HoxD Afc allele.
Keywords		Animals — CRISPR-Cas Systems — Cholecystokinin/genetics — Courtship — Female — Gene Expression — Glutamate Decarboxylase/genetics — Homeodomain Proteins/genetics — In Situ Hybridization — Fluorescence — Male — Mice — Mutation — Neocortex/cytology/metabolism/physiology — Sequence Deletion — Transcription Factors/genetics
Identifiers		DOI: 10.1186/s13104-018-3307-8 PMID: 29580290
Full text		Article (Published version) (4.6 MB) - Free access
Structures		Faculté des sciences / Section de biologie / Département de génétique et évolution
Citation (ISO format)		ZAKANY, Jozsef, DUBOULE, Denis. Rescue of an aggressive female sexual courtship in mice by CRISPR/Cas9 secondary mutation in vivo. In: BMC Research Notes, 2018, vol. 11, n° 1, p. 193. doi: 10.1186/s13104-018-3307-8 https://archive-ouverte.unige.ch/unige:131210