<i>In vivo</i> interaction of the <i>Escherichia coli</i> integration host factor with its specific binding sites

Engelhorn, Manuel; Boccard, Frédéric; Murtin-Borgel, Christine; Prentki, Pierre; Geiselmann, Johannes

doi:10.1093/nar/23.15.2959

Scientific article

English

In vivo interaction of the Escherichia coli integration host factor with its specific binding sites

Contributors

Engelhorn, Manuel; Boccard, Frédéric; Murtin-Borgel, Christine; Prentki, Pierre; Geiselmann, Johannes

Published in

Nucleic Acids Research, vol. 23, no. 15, p. 2959-2965

Publication date

1995

Abstract

The histone-like protein integration host factor (IHF) of Escherichia coll binds to specific binding sites on the chromosome or on mobile genetic elements, and is involved in many cellular processes. We have analyzed the interaction of IHF with five different binding sites In vitro and in vivo using UV laser footprinting, a technique that probes the immediate environment and conformation of a segment of DNA. Using this generally applicable technique we can directly compare the binding modes and Interaction strengths of a DNA binding protein in its physiological environment within the cell to measurements performed in vitro. We conclude that the interactions between IHF and its specific binding sites are identical In vitro and In vivo. The footprinting signal is consistent with the model of IHF-binding to DNA proposed by Yang and Nash(1989). The occupancy of binding sites varies with the concentration of IHF in the cell and allows to estimate the concentration of free IHF protein in the cell.

UNIGE affiliation entities

Faculté des sciences / Section de biologie / Département de biologie moléculaire

Funding

Swiss National Science Foundation - 31-32582.91

Citation (ISO format)

ENGELHORN, Manuel et al. In vivo interaction of the Escherichia coli integration host factor with its specific binding sites. In: Nucleic Acids Research, 1995, vol. 23, n° 15, p. 2959–2965. doi: 10.1093/nar/23.15.2959

Article (Published version)

CC BY

Identifiers

PID : unige:130899
DOI : 10.1093/nar/23.15.2959

Additional URL for this publication

https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/23.15.2959

Journal ISSN

0305-1048

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