en
Scientific article
Open access
English

In vivo interaction of the Escherichia coli integration host factor with its specific binding sites

Published inNucleic Acids Research, vol. 23, no. 15, p. 2959-2965
Publication date1995
Abstract

The histone-like protein integration host factor (IHF) of Escherichia coll binds to specific binding sites on the chromosome or on mobile genetic elements, and is involved in many cellular processes. We have analyzed the interaction of IHF with five different binding sites In vitro and in vivo using UV laser footprinting, a technique that probes the immediate environment and conformation of a segment of DNA. Using this generally applicable technique we can directly compare the binding modes and Interaction strengths of a DNA binding protein in its physiological environment within the cell to measurements performed in vitro. We conclude that the interactions between IHF and its specific binding sites are identical In vitro and In vivo. The footprinting signal is consistent with the model of IHF-binding to DNA proposed by Yang and Nash(1989). The occupancy of binding sites varies with the concentration of IHF in the cell and allows to estimate the concentration of free IHF protein in the cell.

Funding
  • Swiss National Science Foundation - 31-32582.91
Citation (ISO format)
ENGELHORN, Manuel et al. <i>In vivo</i> interaction of the <i>Escherichia coli</i> integration host factor with its specific binding sites. In: Nucleic Acids Research, 1995, vol. 23, n° 15, p. 2959–2965. doi: 10.1093/nar/23.15.2959
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal0305-1048
211views
261downloads

Technical informations

Creation02/18/2020 12:18:00 PM
First validation02/18/2020 12:18:00 PM
Update time03/15/2023 9:09:04 PM
Status update03/15/2023 9:09:03 PM
Last indexation02/12/2024 11:47:50 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack