In vivo interaction of the Escherichia coli integration host factor with its specific binding sites

Article Article scientifique Article In vivo interaction of the Escherichia coli integration host factor with its specific binding sites eng 1995 author Engelhorn Manuel 28868 author Boccard Frédéric 28853 author Murtin-Borgel Christine 24480 author Prentki Pierre 26337 author Geiselmann Johannes 28617 2959-2965 10.1093/nar/23.15.2959 0305-1048 https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/23.15.2959 The histone-like protein integration host factor (IHF) of Escherichia coll binds to specific binding sites on the chromosome or on mobile genetic elements, and is involved in many cellular processes. We have analyzed the interaction of IHF with five different binding sites In vitro and in vivo using UV laser footprinting, a technique that probes the immediate environment and conformation of a segment of DNA. Using this generally applicable technique we can directly compare the binding modes and Interaction strengths of a DNA binding protein in its physiological environment within the cell to measurements performed in vitro. We conclude that the interactions between IHF and its specific binding sites are identical In vitro and In vivo. The footprinting signal is consistent with the model of IHF-binding to DNA proposed by Yang and Nash(1989). The occupancy of binding sites varies with the concentration of IHF in the cell and allows to estimate the concentration of free IHF protein in the cell. Dewey 570 Swiss National Science Foundation 31-32582.91 CH 43 Département de biologie moléculaire 161 false Nucleic Acids Research 23 15 0149b76c-da17-43a0-91c9-7c12a88c96f3 Article (Published version) Public 23-15-2959.pdf 5944363 application/pdf CC BY openAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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In vivo interaction of the Escherichia coli integration host factor with its specific binding sites	Nucleic Acids Research			1995	312	511

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Murtin-Borgel, Christine