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Caprin‐1 Promotes Cellular Uptake of Nucleic Acids with Backbone and Sequence Discrimination

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Published in Helvetica Chimica Acta. 2020, vol. 103, no. e1900255, p. 1-10
Abstract The cellular delivery of oligonucleotides has been a major obstacle in the development of therapeutic antisense agents. PNAs (Peptide Nucleic Acid) are unique in providing a modular peptidic backbone that is amenable to structural and charge modulation. While cationic PNAs have been shown to be taken up by cells more efficiently than neutral PNAs, the generality of uptake across different nucleobase sequences has never been tested. Herein, we quantified the relative uptake of PNAs across a library of 10 000 sequences for two different PNA backbones (cationic and neutral) and identified sequences with high uptake and low uptake. We used the high uptake sequence as a bait for target identification, leading to the discovery that a protein, caprin‐1, binds to PNA with backbone and sequence discrimination. We further showed that purified caprin‐1 added to cell cultures enhanced the cellular uptake of PNA as well as DNA and RNA.
Keywords Peptide nucleic acidsCellular uptakeCaprin-1Antisense agents
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Research groups Groupe Winssinger
Groupe Roux
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GALLI, Valentina et al. Caprin‐1 Promotes Cellular Uptake of Nucleic Acids with Backbone and Sequence Discrimination. In: Helvetica Chimica Acta, 2020, vol. 103, n° e1900255, p. 1-10. doi: 10.1002/hlca.201900255 https://archive-ouverte.unige.ch/unige:129366

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Deposited on : 2020-01-24

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