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Title

A systematic investigation of the effect of sample diluent on peak shape in hydrophilic interaction liquid chromatography
Authors
Ruta, Josephine
Rudaz, Serge
McCalley, David V.
Veuthey, Jean-Luc
Guillarme, Davy
Published in Journal of chromatography. 2010, vol. 1217, no. 52, p. 8230-40
Abstract The aim of this study was to evaluate the importance of sample diluents to improve peak shapes in hydrophilic interaction liquid chromatography (HILIC), using low molecular weight (<1000Da) analytes as well as peptides (with MW ranging between 1000 and 6000Da) as model compounds. Various solvents were tested including water, acetonitrile, methanol, ethanol, propan-2-ol, dimethyl sulfoxide, and a number of combinations of them. For the analysis of small MW compounds, best peak shapes were obtained with sample dissolved in pure ACN but, IPA or a mixture of ACN/IPA (50:50, v/v) could represent a viable alternative in the case of solubility issues with pure ACN. For drug discovery applications, DMSO can be employed but in combination with at least 80% of ACN. For peptides analysis, acetonitrile, EtOH and IPA as sample diluents, provided similar chromatographic profiles, but pure EtOH or IPA were recommended to limit denaturation and samples solubility issues. Finally, whatever the nature of the compounds, it is recommended to add the lowest amount of water to the sample diluent, to maintain suitable peak shapes.
Keywords HILIC — Dissolution solvent — Sample diluent — Peptides — Small compounds — UHPLC
Identifiers
PMID: 21106201
Full text
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Structures
Faculté des sciences / Section des sciences pharmaceutiques
Citation
(ISO format) 		RUTA, Josephine et al. A systematic investigation of the effect of sample diluent on peak shape in hydrophilic interaction liquid chromatography. In: Journal of chromatography, 2010, vol. 1217, n° 52, p. 8230-40. doi: 10.1016/j.chroma.2010.10.106 https://archive-ouverte.unige.ch/unige:12829

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Deposited on : 2010-12-07

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